Homework Answers
Zone of inhibition or disc diffusion method is used to detect the antibiotic sensitivity. In this method first bacteria are spread on the petri plate followed by applying of disc shape antibiotics. After applying of antibiotics standared tinea if incubation is 16 to 18 hours.
If the plates are left at room temperature for periods longer than the standard time. In this condition due to multiplication of the inoculum in place after the discs are applied. Due to this rapid multiplication of inoculum there is reduction in the zone diameter and which may result in reporting of a susceptible strain as resistant strain.
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Kirby-Bauer susceptibility testing method:
What may happen to the zone of inhibition if the plate is...
Kirby-Bauer susceptibility testing method: b. What can happen to the zone of the inhibition if the...
Kirby-Bauer susceptibility testing method:
b. What can happen to the zone of the inhibition if the disks are placed on the plate more than 15 minutes after inoculation?
please asap ty!!! 4. In the Kirby-Bauer susceptibility testing method: (5) a. Why must the standard...
please asap ty!!!
4. In the Kirby-Bauer susceptibility testing method: (5) a. Why must the standard suspension of organism be inoculated within 15 minutes? How will it affect the zone size?
Kirby-Bauer susceptibility testing method: Why can you ignore some organism growth within the zone of the...
Kirby-Bauer susceptibility testing method:
Why can you ignore some organism growth within the zone of the SXT (Trimethoprim/Sulfamethoxazole) disk?
Colonies in the zone represent resistant bacteria. Interpreting the Results of the Bauer Kirby Method of...
Colonies in the zone represent resistant bacteria. Interpreting the Results of the Bauer Kirby Method of Antibiotic Susceptibility Testing Interpreting the Results of the Bauer-Kirby Method of Antibiotic Susceptibility Testing Dr. Gary Kaiser The Community College of Baltimore County Interpreting the Results of the Bauer Kirby Method of Antibiotic Susceptibility Testing
Upon performing a Kirby-Bauer disc diffusion test using Staphylococcus aureus, you observe no zone of inhibition...
Upon performing a Kirby-Bauer disc diffusion test using Staphylococcus aureus, you observe no zone of inhibition with penicillin and a 15mm zone of inhibition with vancomycin. Based on these observation, which of the following conclusions is valid? Staphylococcus aureus is resistant to penicillin and sensitive to vancomycin. Staphylococcus aureus is resistant to both vancomycin and penicillin. Staphylococcus aureus is resistant to penicillin but may or may not be sensitive to vancomycin. Penicillin is bactericidal toward Staphylococcus aureus, and vancomycin is...
1. What does each disc have a precise amount of? SLIDE 3 KIRBY-BAUER ANTIBIOTIC SENSITIVITY PLATE...
1. What does each disc have a precise amount of? SLIDE 3 KIRBY-BAUER ANTIBIOTIC SENSITIVITY PLATE USING MEULLER-HINTON AGAR 2. Does a large zone of inhibition automatically qualify the antibiotic as effective and thus the organisms (sensitive)? 3. How do you know if any one of these zones of inhibition deems the organisms (sensitive) to the antibiotic? 4. What does this agar (MEULLER-HINTON AGAR) control for (how does this agar put all the antibiotics on an equal footing)? 5. Circle...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
Plate 1. Plate 2. MRSA 980 Plate 3. S. aureus MRSA Inhibition (dear)zone Measure in mm...
Plate 1. Plate 2. MRSA 980 Plate 3. S. aureus MRSA Inhibition (dear)zone Measure in mm Figure 2 Katelyn's diagram of how to measure a zone of inhibition from her microbiology lab notebook. Exercise1 Measure the zones of inhibition for each antibiotic on the plates shown in Figure 1 and note the measurements in the spaces in Table 1 below. (Note: The Kirby Bauer method is standardized so that no zone of inhibition is scored as a 0. and all...
Antibiotic Sensitivity Testing 46. What size is the zone of inhibition? mm or less A. 11...
Antibiotic Sensitivity Testing 46. What size is the zone of inhibition? mm or less A. 11 - B. 12-13 mm - C. 14 mm or more 47.Is this organism sensitive, resistant or intermediate to the ampicillin? - A. sensitive B. resistant - C. intermediate Spring 2018
Kirby-Bauer susceptibility testing method:
b. What can happen to the zone of the inhibition if the disks are placed on the plate more than 15 minutes after inoculation?
please asap ty!!!
4. In the Kirby-Bauer susceptibility testing method: (5) a. Why must the standard suspension of organism be inoculated within 15 minutes? How will it affect the zone size?
Kirby-Bauer susceptibility testing method:
Why can you ignore some organism growth within the zone of the SXT (Trimethoprim/Sulfamethoxazole) disk?
Colonies in the zone represent resistant bacteria. Interpreting the Results of the Bauer Kirby Method of Antibiotic Susceptibility Testing Interpreting the Results of the Bauer-Kirby Method of Antibiotic Susceptibility Testing Dr. Gary Kaiser The Community College of Baltimore County Interpreting the Results of the Bauer Kirby Method of Antibiotic Susceptibility Testing
1. What does each disc have a precise amount of? SLIDE 3 KIRBY-BAUER ANTIBIOTIC SENSITIVITY PLATE USING MEULLER-HINTON AGAR 2. Does a large zone of inhibition automatically qualify the antibiotic as effective and thus the organisms (sensitive)? 3. How do you know if any one of these zones of inhibition deems the organisms (sensitive) to the antibiotic? 4. What does this agar (MEULLER-HINTON AGAR) control for (how does this agar put all the antibiotics on an equal footing)? 5. Circle...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
Plate 1. Plate 2. MRSA 980 Plate 3. S. aureus MRSA Inhibition (dear)zone Measure in mm Figure 2 Katelyn's diagram of how to measure a zone of inhibition from her microbiology lab notebook. Exercise1 Measure the zones of inhibition for each antibiotic on the plates shown in Figure 1 and note the measurements in the spaces in Table 1 below. (Note: The Kirby Bauer method is standardized so that no zone of inhibition is scored as a 0. and all...
Antibiotic Sensitivity Testing 46. What size is the zone of inhibition? mm or less A. 11 - B. 12-13 mm - C. 14 mm or more 47.Is this organism sensitive, resistant or intermediate to the ampicillin? - A. sensitive B. resistant - C. intermediate Spring 2018
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