Homework Answers
Mutant - Lac I code for repressor molecule which bind on operator and off lac operon in absence of lactose.
If i gene is mutated, all the time expressions of lac will turn on so Z Expressions will occur all the time.
Mutant 2, Lac o- is region where repressor bind in absence of lactose so if it is mutated, then no repressor can bind on it and expressions of all genes including Z remain turn on.
Mutant 3, In this case mutation is in A gene and it has on effect on expression of Z gene.
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3. The following table lists several possible null (gene-inactivating) mutations in the lac operon system. Fill...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl E. coli cells. These have two copies of each of the lac operon genes, one in their chromosome and one on a plasmid* (actually a separate chromosome fragment introduced via conjugation), so they are "partial diploids." Many different versions of these were constructed, with different combinations of mutations in the chromosomal and/or plasmid- encoded genes. +/- indicate active/inactive gene products. O® = "constitutively active...
Imagine you are carrying out research on the lac operon. You isolate six mutations in the...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
Microbiology question 51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for...
Microbiology question
51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for lactose metabolism. The genotypes of different E. coli strains are listed below. (For this exercise, lacP refers to the promoter, and lacO refers to the operator site where Lacl binds. lacl+ means wild-type, whereas lacl- indicates a loss-of-function mutation. lacO- means that Lacl cannot bind the operator.) For each strain, indicate whether the LacZ protein will be synthesized under the conditions indicated, when glucose...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts the function of each of the following elements: a. the promoter for LacI (P(I)) b. the LacI gene c. CRP binding site d. the promoter for the lac operon (P(lac)) e. the operator sequence f. a mutation in lacZ that disrupts the coding region but does not disrupt transcription g. a mutation in lacZ that blocks transcription For each of the above mutations, what...
The lac operon consists of a promoter that initiates transcription of the gene i, which encodes...
The lac operon consists of a promoter that initiates
transcription of the gene i, which encodes a repressor protein. A
seperate promoter, p, within the operon initiates transcription of
z, which encodes B-galactosidase, and y, which encodes permease.
The operator, o, where the repressor protein binds to inhibit
transcription, is positioned between mutations, whereas superscript
c denotes a constitutively active mutant. The table below sea fives
the activity of B-galatosidase and permease for haploid and diploid
mutant strains. Match the...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
B2. Consider E. coli cells, each having one of the following mutations: a) a mutant lac...
B2. Consider E. coli cells, each having one of the following mutations: a) a mutant lac operator (Oc locus) that cannot bind repressor. b) a mutant lac repressor (I- gene product) that cannot bind to the lac operator. c) a mutant lac repressor (the Is gene product) that cannot bind to lactose. d) a mutant lac promoter that cannot bind CAP + cAMP. What effect would each mutation have on the function of lac operon (assuming no glucose is present)...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl E. coli cells. These have two copies of each of the lac operon genes, one in their chromosome and one on a plasmid* (actually a separate chromosome fragment introduced via conjugation), so they are "partial diploids." Many different versions of these were constructed, with different combinations of mutations in the chromosomal and/or plasmid- encoded genes. +/- indicate active/inactive gene products. O® = "constitutively active...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
Microbiology question
51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for lactose metabolism. The genotypes of different E. coli strains are listed below. (For this exercise, lacP refers to the promoter, and lacO refers to the operator site where Lacl binds. lacl+ means wild-type, whereas lacl- indicates a loss-of-function mutation. lacO- means that Lacl cannot bind the operator.) For each strain, indicate whether the LacZ protein will be synthesized under the conditions indicated, when glucose...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
The lac operon consists of a promoter that initiates
transcription of the gene i, which encodes a repressor protein. A
seperate promoter, p, within the operon initiates transcription of
z, which encodes B-galactosidase, and y, which encodes permease.
The operator, o, where the repressor protein binds to inhibit
transcription, is positioned between mutations, whereas superscript
c denotes a constitutively active mutant. The table below sea fives
the activity of B-galatosidase and permease for haploid and diploid
mutant strains. Match the...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
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