Question

Commercial Salmonella O antigen or Salmonella enterica Typhimurium (heated for 30 minutes at 56°C in a water bath)

+=positive control

-=Negative control

A= sample of protein

B= sample of protein

ut --- AAA B BB

What are the possible results and how can they be interpreted?

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Answer #1

Salomonella O antigen is a antigen that protect the organism from complement system. according to this figure it got agglutinated with positive control and A sample of protein. That is the given antibodies have ability to agglutinate the antigen . That is in both tests, the insoluble form of antigen form complex with soluble form of antibody forming blue coloured clumps or agglutinates. the given serum protein A contains antibodies against the bacteria.

The negative control and Serum protein B lacks antibodies against antigen produced by the bacteria hence less resistant to disease caused by the organism.

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