You have a 1M stock solution of glucose and you want to prepare three separate dilutions:...

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You have a 1M stock solution of glucose and you want to prepare three separate dilutions:...

You have a 1M stock solution of glucose and you want to prepare three separate dilutions: 100mM, 10mM, and 0.1mM glucose. How would you do this?

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Answer 1

Answer #1

1M glucose is the stock solution, to prepare dilutions of 100mM, 10mM and 0.1mM serial dilutions can be made or direct dilutions can be done as follows

100mM: A ten times dilutions is required to use 1M stock: 10ml of 1M glucose stock + 90ml of water/buffer = 100mM glucose

10mM: two methods are available to make this

1. Use a 1M stock and make 100 times dilution: 1ml of 1M glucose stock + 99ml of water/buffer = 10mM glucose

2. Use 100mM stock obtained in the first dilution: 1ml of 100mM glucose stock + 9ml of water/buffer = 10mM glucose

0.1mM : two methods are available to make this

1. Use a 1M stock and make 1000 times dilution: 1ml of 1M glucose stock + 999ml of water/buffer = 10mM glucose

2. Use 10mM stock obtained in the second dilution: 1ml of 10mM glucose stock + 99ml of water/buffer = 10mM glucose

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Answer 2

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