What can you conclude about a DNA sample that has an a260/a280 ratio of 2.10 and...
What can you conclude about a DNA sample that has an a260/a280 ratio of 2.10 and an a260/a230 ratio of 1.70
Homework Answers
After the isolation of the DNA we will check the purity of the DNA by nanodrop.
At 260/280 the DNA sample concentration should be around 1.8 but in this case the concentration is 2.10 which is a bit high and it is contaminated during isolation.
Where the ratio of 230 should be high than the 280 in this case 230 value is less than the 280 due to which there is more contamination.
At last we conclude that the DNA sample purity is very less and cannot go further process using this DNA.
Add Answer to:
What can you conclude about a DNA sample that has an a260/a280
ratio of 2.10 and...
Can you pleasee answer these questions regarding the experiment of DNA quantification. What is “blank” and...
Can you pleasee answer these questions regarding the experiment of DNA quantification. What is “blank” and why do we use it in DNA quantification? If you are told to analyze DNA samples that we extracted from buccal swabs, what would you use as a blank? 3. Explain in details: a. Concentration b. A260/A280 ratio c. A260/A230 Why are those measurements used, how do you know which concentration to expect if you used the commercial kit for DNA extraction, what is...
Q5: (2) DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain...
Q5: (2) DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain the values listed below. The DNA had been diluted 10 fold. Remember that a solution of DNA that is 50μg/ml (or 50ng/μl) has an absorbance of 1.0 at a wavelength of 260 nm. What is the concentration of the DNA in this sample (In this question DO NOT ignore the problem of dust in the sample and give answer in ng/ul). A260 = 0.80...
"calculate the total mass of DNA that you isolated at the end of Part A" how...
"calculate the total mass of DNA that you isolated at the end of Part A" how would you go about doing this, the concentration in my diluted DNA sample was 6.3ug/ml, and my A60 value was 0.126 Abs i don't know if these numbers help.. part A I had to calculate the concentration of DNA in my undiluted sample to which I did Concentration (ug/mL) = (A260 – A280) dilution factor 50ug/mL C=0.126-0.060 20 50nguL= 66ng/uL and go that the concentration...
Given values A260 value of the diluted sample = 0.126 Abs the concentration of DNA in...
Given values A260 value of the diluted sample = 0.126 Abs the concentration of DNA in my diluted sample = 6.3ug/ml Dilution factor = 20 MY QUESTION Calculate the concentration of DNA in your undiluted sample - (I got 126ng/ul as an answer but unsure if this is right) calculate the total mass of DNA you isolated *** show work for this pls
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260...
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260 nm) of DNA solution using a spectrophotometer. You took 10 µl of your plasmid DNA preparation and dilute with 990 µl of H2O. The A260of this diluted solution was 0.30. Calculate the concentration of your plasmid DNA preparation (before the dilution). It has been established that 50 µg/ml of double-stranded DNA gives A260 = 1.0. My plasmid DNA preparation is _____________________ mg/ml. From this...
Cac), Towever, h most applications it is advantageous to store DNA in TE Buffer (10mM Tris...
Cac), Towever, h most applications it is advantageous to store DNA in TE Buffer (10mM Tris with 1mM EDTA). Why is it problematic in this experiment to resuspend the DNA in TE buffer? 6. Assume that a 50 μg/mL solution of native DNA has an A260 of 1, calculate the concentration of your DNA sample in mg/mL. Then, assuming that 10% of the onion cells mass are the nuclei and that 10% of the nuclei mass is DNA, calculate the...
When analyzing financial statements, what can you conclude when the inventory turnover ratio increases from 4.0...
When analyzing financial statements, what can you conclude when the inventory turnover ratio increases from 4.0 to 6.0 over a three year period. The day’s inventory held are within the typical industry average The day’s inventory held has increased over time The day’s inventory held has decreased over time When analyzing financial statements, what can you conclude when the accounts receivable turnover ratio decreases from 9.0 to 6.0 over a three year period. Collections are within standard terms The collection...
Can someone help me interpret our reading into context and answer the following questions related. Proteins...
Can someone help me interpret our reading into context and
answer the following questions related.
Proteins and phenol absorb ultraviolet light. Both have absorption peaks between 260 and 300 nm. What would contamination with protein and/or phenol do to the ratio A260/A280? If your DNA solution was contaminated with protein and/or phenol, what would happen to the peak of the absorption curve (relative to the peak of a curve for pure RNA/DNA)? Does your DNA-solution seem to be contaminated with...
the electric potential at a point is large, what can you conclude about the behavior of...
the electric potential at a point is large, what can you conclude about the behavior of the electric field there?
The conjugate acid of ethanol amine (HOCH2CH2NH3+) has pKa = 9.50. What can you conclude about...
The conjugate acid of ethanol amine (HOCH2CH2NH3+) has pKa = 9.50. What can you conclude about a solution of this amine in which [RNH3+)] = 10 x [RNH2]?) Question 9 options: a) pH = 10.50 b) pH = 9.50 c) pH = 8.50 d) None of the above.
Cac), Towever, h most applications it is advantageous to store DNA in TE Buffer (10mM Tris with 1mM EDTA). Why is it problematic in this experiment to resuspend the DNA in TE buffer? 6. Assume that a 50 μg/mL solution of native DNA has an A260 of 1, calculate the concentration of your DNA sample in mg/mL. Then, assuming that 10% of the onion cells mass are the nuclei and that 10% of the nuclei mass is DNA, calculate the...
Can someone help me interpret our reading into context and
answer the following questions related.
Proteins and phenol absorb ultraviolet light. Both have absorption peaks between 260 and 300 nm. What would contamination with protein and/or phenol do to the ratio A260/A280? If your DNA solution was contaminated with protein and/or phenol, what would happen to the peak of the absorption curve (relative to the peak of a curve for pure RNA/DNA)? Does your DNA-solution seem to be contaminated with...
Most questions answered within 3 hours.
-
What major physiological changes account for electrodermal
activity (as in during a lie-detector test)?
asked 34 minutes ago -
You are going to sell fruits & vegetables at a local
roadside market. List separately and...
asked 1 hour ago -
Hows does water affect the digestion of fats ?
asked 3 hours ago -
A buffer that contains 0.41 M of a base, B and 0.21 M of its
conjugate...
asked 3 hours ago -
Pleaaaaaaaasssssse help me please please it's more important
:((((((((((((((((((((
CASE STUDY
Nissan Company
Nissan Motor Company,...
asked 3 hours ago -
Calculate the concentration of hydronium and hyroxide ions in 1)
2.0 mol/L HNO3 2) 0.5 mol/L...
asked 4 hours ago -
In a loop-the-loop ride a car goes around a vertical, circular
loop at a constant speed....
asked 4 hours ago -
50 grams each of water (18.015 g/mol) and ethylene glycol
(C2H6O2) (62.07 g/mol) are mixed. At...
asked 4 hours ago -
The life expectancy of sport utility vehicles follows a normal
distribution with a mean of 145.8...
asked 4 hours ago -
If an object with mass of 5 kg is pushed with a force of 552 N,...
asked 4 hours ago -
Suppose your program already has a list called nums, which
contains several numbers. Write a for...
asked 4 hours ago -
A spherical shell with a finite thickness just barely floats in
water. If the shell is...
asked 4 hours ago