Answer :-
The word disinfectant is used for an agent used to disinfect
items or structures that are inanimate but is usually harmful to
human tissue.
1. The Chemical Agent Concentration.
2.The temperature the agent is in use at. In general, the longer it
takes to disinfect or decontaminate the lower the
temperature.
3. The latest type of microorganisms. It is more difficult to
eliminate Endospore users, such as species Bacillus, species
Clostridium, and acid-fast bacteria such as Mycobacterium
tuberculosis.
4. Number of living microorganisms. The more microorganisms
present, the more difficult it becomes to disinfect or
decontaminate.
5. The nature of the microorganisms carrying the material. Organic
substance including soil and excreta interfers with certain
agents.
An rise in pH increases some disinfectants ' antimicrobial activity (e.g., glutaraldehyde), but reduces others ' antimicrobial activity (e.g., phenols).
Water hardness reduces the kill rate of certain disinfectants because divalent cations (e.g. calcium) bind to insoluble precipitates with the disinfectant in the hard water.
the disinfecting ability of a substance determined by dividing the
figure indicating the degree of disinfectant dilution which kills a
microorganism in a given time by indicating the degree of phenol
dilution which kills the microorganism under similar
conditions.
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