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O words 2.5 pts Question 4 What are some factors that can alter the effectiveness of a disinfectant? (Refer to the disk-diffu
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The word disinfectant is used for an agent used to disinfect items or structures that are inanimate but is usually harmful to human tissue.
1. The Chemical Agent Concentration.
2.The temperature the agent is in use at. In general, the longer it takes to disinfect or decontaminate the lower the temperature.
3. The latest type of microorganisms. It is more difficult to eliminate Endospore users, such as species Bacillus, species Clostridium, and acid-fast bacteria such as Mycobacterium tuberculosis.
4. Number of living microorganisms. The more microorganisms present, the more difficult it becomes to disinfect or decontaminate.
5. The nature of the microorganisms carrying the material. Organic substance including soil and excreta interfers with certain agents.

An rise in pH increases some disinfectants ' antimicrobial activity (e.g., glutaraldehyde), but reduces others ' antimicrobial activity (e.g., phenols).

Water hardness reduces the kill rate of certain disinfectants because divalent cations (e.g. calcium) bind to insoluble precipitates with the disinfectant in the hard water.


the disinfecting ability of a substance determined by dividing the figure indicating the degree of disinfectant dilution which kills a microorganism in a given time by indicating the degree of phenol dilution which kills the microorganism under similar conditions.

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