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In class we discussed a model showing ATP binding for the purpose of hydrolysis. Answer the...

In class we discussed a model showing ATP binding for the purpose of hydrolysis. Answer
the following:
a. What is the role of Mg2+?
b. What would happen if the Walker-A Threonine and Walker-B Aspartate were mutated to Alanines?
c. What is the role of the Arginine finger?

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Answer #1

The magnesium ion bind with the beta and gamma phosphate atoms with oxygen atoms which take the electron density of phosphate groups and thus makes the Molecules less electronegative .

This helps in the nucleophilic attach to the phosphate atoms and thus hydrolysis of ATP.

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