Question

This DNA sequence represents an open reading frame (ORF) of a transcriptional unit. Transcribe and then...

This DNA sequence represents an open reading frame (ORF) of a transcriptional unit. Transcribe and then translate this gene in the spaces provided below.

5' ATGGGAGCTGTTGTATTTGA 3'

3' TACCCTCGAGCAACATAAACT 5'

Transcribe mRNA sequence and translated protein sequence.

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Answer #1

The given DNA sequence (ORF) is

5' ATGGGAGCTCGTTGTATTTGA 3'

3' TACCCTCGAGCAACATAAACT 5'

So, after transcription of the template strand (3' TACCCTCGAGCAACATAAACT 5') the resultant mRNA will be as following

5' AUGGGAGCUCGUUGUAUUUGA 3'

Consequently, after the translation of the mRNA, the amino acid sequence of translated protein will be as following

Met(Start) - Gly - Ala - Arg - Cys - Ile - (Stop)

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