Question

I. Use the DNA sequence below, which encodes a prokaryotic gene to answer the following questions. 11 21 31 41 51 71 81 61 91 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA 101 111 121 131 141 ACCCCCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT a) Write down the corresponding sequences, find them in the sequence above and label them: -35 Consensus sequence: _(label as -35) -10 Consensus sequence (Pribnow box): _ __ (label as Pribnow) Shine-Dalgarno sequence in corresponding mRNA: __ (label as SD) Start (initiation) codon in corresponding mRNA: (label as start) Stop (termination) codon in corresponding mRNA: (label as stop) b) What region of this prokaryotic DNA sequence will be transcribed into mRNA? Circle one. 1-131 71-11974-14954-119 c) What will the sequence be for the protein translated from this mRNA? d) Where is the 3'UTR? Circle one. 9-131 41-119 81-141 87-119 II. Use the DNA sequence below, which encodes a eukaryotic gene to answer the following questions. 11 21 31 41 GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA TGGACGCGCC 51 61 91 ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA GTCATTCAGC 101 111 121 GTAGTCTGAT GCCAGTCGAC TGC 71 81 a) Write down the corresponding sequences, find them in the sequence above and label them: TATA box sequence: _ (label as TATA) Polyadenylation signal in corresponding mRNA: (label as Poly-A) Kozak's sequence in corresponding mRNA: (label as Kozak) Start (initiation) codon in corresponding mRNA: (label as start) Stop (termination) codon in corresponding mRNA: _ (label as stop) b) What region of the mRNA contains the open reading frame that will be translated into protein? 41-101 27-35 51-59 7 1-77 c) Where is the 3'UTR? Circle one. 59-101 5 1-123 63-101 71-81

Answer 1

. Use the DNA sequence below, which encodes a prokaryotic gene to answer the following questions. -35 sequence pribnow box 41 1 11 21 SD 31 ATGAGGAGTT GACACACAAG AGGAGGTAGC AGTATGGGTA TAATCTAATG Start 51 61 71 81 91 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA Stop 121 Start 101 111 131 Stop 141 ACCCCCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT a) Write down the corresponding sequences, find them in the sequence above and label them: -35 Consensus sequence: (label as -35) -10 Consensus sequence (Pribnow box): Shine-Dalgarno sequence in corresponding mRNA Start (initiation) codon in corresponding MRNA: Stop (termination) codon in corresponding MRNA:) (label as Pribnow) (label as SD) (label as start) (label as stop) b) What region of this prokaryotic DNA sequence will be transcribed into MRNA? Circle one. 1-131 71-119 74-149 54-119 c) What will the sequence be for the protein translated from this mRNA? 5' Met-Asn- ser 3 5' Met-Arg-Asn 3' d) Where is the 3'UTR? Circle one. 9-131 41-119 81-141 87-119 II. Use the DNA sequence below, which encodes a eukaryotic gene to answer the following questions 31 1 11 21 41 TATA GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA TGGACGCGCC kozak 51 61 71 81 91 ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA GTCATTCAGC 121 Poly A stopl 11 GTAGTCTGAT GCCAGTCGAC TGC a) Write down the corresponding sequences, find them in the sequence above and label them: TATA box sequence: (label as TATA) (label as Poly-A) (label as Kozak) |(label as start) (label as stop) Polyadenylation signal in corresponding MRNA: Kozak's sequence in corresponding MRNA: Start (initiation) codon in corresponding MRNA: Stop (termination) codon in corresponding MRNA: b) What region of the MRNA contains the open reading frame that will be translated into protein? 41-101 27-35 51-59 71-77 Where is the 3'UTR? Circle one c) 51-123 63-101 59-101 71-81