Imagine you have created multiple standard curves for organism A and are now confident that the...
Imagine you have created multiple standard curves for organism A and are now confident that the OCD of organism A can be accurately estimated just by taking the OD at 600nm. You now need to grow the same organism in a new medium. Can you use the same standard curve, why or why not?
Homework Answers
The same standard growth curve cannot be used for the organism for different media due to following reasons,
Typically the growth curve has four distinct growth phases:
1. Lag Phase
2. Exponential Phase
3. Stationary Phase
4. Death Phase
These phases may have differences because of media components like pH, Carbon and nitrogen source, shaking condition, oxygen level etc. Also, there could be variation in OD because of color or density of media.
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