Question

Write neat please and box the final answer please

Question 5 0.5 pts Before the lagging strand can begin assembling new DNA nucleotides, which of the following must occur? OH bonds must be broken between the two strands of DNA DNA polymerase I removes some material and replaces it with DNA ORNA primase constructs a short RNA primer. DNA Ligase forms a phosphodiester bond between the 3'OH of the growing strand and the 5' phosphate in front of it. DNA polymerase III adds deoxyribonucleotides Question 6 0.5 pts The nicks, or gaps, between fragments on the lagging strand are eventually sealed by DNA ligase RNA ligase DNA gyrase Restriction endonucleases

Answer 1

Q 1. DNA replication is a process where replica of DNA is produced from the parent DNA. Replication requires the DNA to separate the strands. In double helix ,one straand acts as leading strand where as another strand act as lagging strand. Lagging strand undergo replication discontinuously in small fragments. So before lagging strand can begin assembling new DNA nucleotides, primer must be present in order to start the replication. Thats why RNA primase must construct a short primer prior to this process. So the answer is option C.

Q 2. DNA ligase seals the the small fragments / okazaki fragments formed during the replication of double stranded DNA .So the answer is option A.