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if the 100% transduction of leu A mutant allele in wild type ecoli, and grow in minimal glucose plate. all the colony of e.coli contain mutant allele of TetR (-leu A). because all bacteria transform and contain mutant allel.
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10 points. An E. coli strain with a Tn10 that is 75% linked to a leuA...
10 points. An E. coli strain with a Tn10 that is 45% linked to the wt...
10 points. An E. coli strain with a Tn10 that is 45% linked to the wt proВ locus (Pro+) is used to transduce an E. coli strain which contains a mutant proВ allele (Pro-) to TetR on rich Tet plates. If 100 transductants are scored on minimal glucose Tet plates how many will be TetR Pro+ and how many will be TetR Pro-?
10 points. The thr and leu operons are 55% linked in the E. coli chromosome. A...
10 points. The thr and leu operons are 55% linked in the E. coli chromosome. A P1 lysate from a thr-leu+ strain is used to transduce a thr+ leu- strain to leu+ using a minimal glucose plate. What possible recombinants can be obtained (specify them as thr+/- leu +/-)? 10 points. An E. coli strain with a Tn10 that is 75% linked to a leu A mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying Tn10 A and the culture was plated on LB-tet media to obtain tet-resistant colony forming units. (LB is a rich growth media for E. coli and contains all amino acids required for bacterial growth) Individual CFU were then transferred to a multiwell plate containing liquid LB-tet media and...
consider a strain of E. coli that has a mutation in the gene encoding the IIA...
consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E....
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E. coli to a thr(-)bio(-)thi(-) recipient strain. When transductants selected on a medium containing thiamine and threonine are tested for growth on other media, many more colonies are found on media supplemented only with threonine than on media supplemented only with thiamine. No transductants survive on minimal media. What is the correct order for these three genes? *I have seen multiple different answers for this,...
1. An auxotrophic E. coli strain requires adenine to grow because of a mutation in a...
1. An auxotrophic E. coli strain requires adenine to grow because of a mutation in a gene for an adenine synthesis enzyme. The following shows part of the wild-type and mutant alleles of the gene, including the start codon. The bottom strand is the template for transcription. How does this mutation affects the enzyme? oder wild- type allele ode mutant allele S'...TTATGGGCAAGATCCCA... 3 3...AATACCCGTTCTAGGGT... 5 ... TTATGGGCTAGATCCCA...3 3...AATACCCGATCTAGGGT...5
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli...
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli which is cys+ ton trp- to infect a recipient strain of E. coli which is cys- tons trp+. These bacteria are then plated on complete media with phage T1 (tons sensitive to phage T1). You obtain 50 ton? transductants which you go on to test on minimal media for the other markers. cyst trp+ cyst trp- cys- trpt cys- trp- 10 24 13 a....
QUESTION 10 A geneticist has discovered a mutant E. coli strain that is normal at 37°C....
QUESTION 10 A geneticist has discovered a mutant E. coli strain that is normal at 37°C. However, when grown at 40°C, all functional protein synthesis stops. The geneticist finds out that at 40°C ribosomes are associated with mRNA, but only a single charged tRNA is associated with the ribosome. What is the most likely problem with this E. coli strain? O mutant peptidyl transferase O mutant Shine-Dalgarno sequences O mutant EF-G O mutant EF-Tu mutant 1F3 factors O mutant RF1
10 points. An E. coli strain with a Tn10 that is 45% linked to the wt proВ locus (Pro+) is used to transduce an E. coli strain which contains a mutant proВ allele (Pro-) to TetR on rich Tet plates. If 100 transductants are scored on minimal glucose Tet plates how many will be TetR Pro+ and how many will be TetR Pro-?
10 points. The thr and leu operons are 55% linked in the E. coli chromosome. A P1 lysate from a thr-leu+ strain is used to transduce a thr+ leu- strain to leu+ using a minimal glucose plate. What possible recombinants can be obtained (specify them as thr+/- leu +/-)? 10 points. An E. coli strain with a Tn10 that is 75% linked to a leu A mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying Tn10 A and the culture was plated on LB-tet media to obtain tet-resistant colony forming units. (LB is a rich growth media for E. coli and contains all amino acids required for bacterial growth) Individual CFU were then transferred to a multiwell plate containing liquid LB-tet media and...
consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
1. An auxotrophic E. coli strain requires adenine to grow because of a mutation in a gene for an adenine synthesis enzyme. The following shows part of the wild-type and mutant alleles of the gene, including the start codon. The bottom strand is the template for transcription. How does this mutation affects the enzyme? oder wild- type allele ode mutant allele S'...TTATGGGCAAGATCCCA... 3 3...AATACCCGTTCTAGGGT... 5 ... TTATGGGCTAGATCCCA...3 3...AATACCCGATCTAGGGT...5
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli which is cys+ ton trp- to infect a recipient strain of E. coli which is cys- tons trp+. These bacteria are then plated on complete media with phage T1 (tons sensitive to phage T1). You obtain 50 ton? transductants which you go on to test on minimal media for the other markers. cyst trp+ cyst trp- cys- trpt cys- trp- 10 24 13 a....
QUESTION 10 A geneticist has discovered a mutant E. coli strain that is normal at 37°C. However, when grown at 40°C, all functional protein synthesis stops. The geneticist finds out that at 40°C ribosomes are associated with mRNA, but only a single charged tRNA is associated with the ribosome. What is the most likely problem with this E. coli strain? O mutant peptidyl transferase O mutant Shine-Dalgarno sequences O mutant EF-G O mutant EF-Tu mutant 1F3 factors O mutant RF1
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