Homework Answers
The linkage of two loci is a measure of the extent to which the two loci would be inherited together. In terms of transduction, it is a frequency at which the second loci will be inherited given the first loci is transferred.
Here we have a transposon Tn10 which is linked to proB locus at a linkage of 45%. Thus, when Tn10 is transferred via transduction, there is a 45% probability that proB will also be transferred to the recipient. Here, the donor is proB+ while the recipient is proB-. Thus, if the recipient receives proB from the donor, it will also become proB+.
There are 100 transductants in the sample. Since there is a 45% linkage, there is a 45% chance that Tn10 will be transduced alongwith the proB. Thus 45% of the recipients will receive proB and will become proB+. Thus, 45 transductants will be proB+ while 55% will be proB-.
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10 points. An E. coli strain with a Tn10 that is 45% linked to the wt...
10 points. An E. coli strain with a Tn10 that is 75% linked to a leuA...
10 points. An E. coli strain with a Tn10 that is 75% linked to a leuA mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich Tet plates. If 100 of the transductants are scored on minimal glucose Tet plates how many will be TetR Leu+ and how many will be TetR Leu-?
10 points. The thr and leu operons are 55% linked in the E. coli chromosome. A...
10 points. The thr and leu operons are 55% linked in the E. coli chromosome. A P1 lysate from a thr-leu+ strain is used to transduce a thr+ leu- strain to leu+ using a minimal glucose plate. What possible recombinants can be obtained (specify them as thr+/- leu +/-)? 10 points. An E. coli strain with a Tn10 that is 75% linked to a leu A mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying Tn10 A and the culture was plated on LB-tet media to obtain tet-resistant colony forming units. (LB is a rich growth media for E. coli and contains all amino acids required for bacterial growth) Individual CFU were then transferred to a multiwell plate containing liquid LB-tet media and...
consider a strain of E. coli that has a mutation in the gene encoding the IIA...
consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the...
genetic question ASAP!!! (5) (10 0 points) In re ser pro* his ret cells are ser...
genetic question ASAP!!!
(5) (10 0 points) In re ser pro* his ret cells are ser pro his (i.e. they will only grow if serine, proline and histidine are included in ne or h and the recipient cells are ser pro the media a generalized transduction system using P1 phage, the E. coli donor cells a transductants on re then tested on plates containing just minimal medihedonor allele ser is initially selected after transduction (by growing potential tras edia plus...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
1. An auxotrophic E. coli strain requires adenine to grow because of a mutation in a...
1. An auxotrophic E. coli strain requires adenine to grow because of a mutation in a gene for an adenine synthesis enzyme. The following shows part of the wild-type and mutant alleles of the gene, including the start codon. The bottom strand is the template for transcription. How does this mutation affects the enzyme? oder wild- type allele ode mutant allele S'...TTATGGGCAAGATCCCA... 3 3...AATACCCGTTCTAGGGT... 5 ... TTATGGGCTAGATCCCA...3 3...AATACCCGATCTAGGGT...5
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E....
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E. coli to a thr(-)bio(-)thi(-) recipient strain. When transductants selected on a medium containing thiamine and threonine are tested for growth on other media, many more colonies are found on media supplemented only with threonine than on media supplemented only with thiamine. No transductants survive on minimal media. What is the correct order for these three genes? *I have seen multiple different answers for this,...
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm...
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm Tet' gene pxyls HOC 5 G. E. coli Amp' gene Kan gene Mutants of this transformed E. coli strain can be obtained by exposing the cells to dimethyl sulfate. Briefly, explain how you might screen these mutants to obtain an xyls variant that can bind to 4-ethylbenzoic acid 1, carefully explaining the logic underlying your protocol. You can assume that wild type xyls cannot...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
10 points. An E. coli strain with a Tn10 that is 75% linked to a leuA mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich Tet plates. If 100 of the transductants are scored on minimal glucose Tet plates how many will be TetR Leu+ and how many will be TetR Leu-?
10 points. The thr and leu operons are 55% linked in the E. coli chromosome. A P1 lysate from a thr-leu+ strain is used to transduce a thr+ leu- strain to leu+ using a minimal glucose plate. What possible recombinants can be obtained (specify them as thr+/- leu +/-)? 10 points. An E. coli strain with a Tn10 that is 75% linked to a leu A mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying Tn10 A and the culture was plated on LB-tet media to obtain tet-resistant colony forming units. (LB is a rich growth media for E. coli and contains all amino acids required for bacterial growth) Individual CFU were then transferred to a multiwell plate containing liquid LB-tet media and...
consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the...
genetic question ASAP!!!
(5) (10 0 points) In re ser pro* his ret cells are ser pro his (i.e. they will only grow if serine, proline and histidine are included in ne or h and the recipient cells are ser pro the media a generalized transduction system using P1 phage, the E. coli donor cells a transductants on re then tested on plates containing just minimal medihedonor allele ser is initially selected after transduction (by growing potential tras edia plus...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
1. An auxotrophic E. coli strain requires adenine to grow because of a mutation in a gene for an adenine synthesis enzyme. The following shows part of the wild-type and mutant alleles of the gene, including the start codon. The bottom strand is the template for transcription. How does this mutation affects the enzyme? oder wild- type allele ode mutant allele S'...TTATGGGCAAGATCCCA... 3 3...AATACCCGTTCTAGGGT... 5 ... TTATGGGCTAGATCCCA...3 3...AATACCCGATCTAGGGT...5
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm Tet' gene pxyls HOC 5 G. E. coli Amp' gene Kan gene Mutants of this transformed E. coli strain can be obtained by exposing the cells to dimethyl sulfate. Briefly, explain how you might screen these mutants to obtain an xyls variant that can bind to 4-ethylbenzoic acid 1, carefully explaining the logic underlying your protocol. You can assume that wild type xyls cannot...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
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