Question

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Question #4 (10 points)-Mass spectroscopy Amino Acid Molecular Weight 89 174 133 146 75 146 115 137, 194 Alanine Arginine Aspartic acid Glutamic acid Glycine Lysine Proline Serine Tyrosine Valine 425, 1051 384 340 619 146 105 891 473 5301 2701 181 117 Mass/charge (here the charge is - 1. so it's just mass) Above is shown a mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this peptide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) The masses given should be less by one (loss of Hion to impose a charge on the molecules). To avoid any possible confusion, I left that out.

it is biochemistry question not the chemistry. By mistake I put chemistry. please do it and send me answer as soon as possible, thank youuuu.

Answer 1

Solution

The pentspeptide sequence is

N-Glutamate-Aspartate-Lysine-Serine-Arginine-C

because all 5 amino acid matching the peak value to its molecular weight.

Glutamate showing mol.wt.of 146 but actual mol.wt. is 147. So if we want to identify N terminus of pentapeptide we have add mol.wt. of 1 Hydrogen into glutamate. Hence glutamate present at N terminus position.

according to stablization of positive and negative charged amino acids if negatively charged amino acid is present at first position then positively charged amino acid will be far away by 3/4 amino acid apart from negatively charged amino acid.

so arginine present at C terminus of pentapeptide.

aspartate can present at 2nd position and lysine and serin will at 3 and 4th position respectively.