ANSWER
An enzyme biosensor is an analytical device that combines an enzyme with a transducer to produce a signal proportional to target analyte concentration. ... This signal can be further amplified, processed, or stored for later analysis.
A Hydrogen peroxide (H2O2) biosensor, based on hemoglobin (Hb) and ortho-phenylenediamine (o-PD) gold electrode, was fabricated. Hb was immobilized onto the electrode surface by electrochemical polymerize method with o-PD. The designed biosensor showed a well defined redox peak which was attributed to the direct electrochemical response of Hb. The immobilized Hb exhibited an excellent electrocatalytical response to the reduction of hydrogen peroxide, enabling the sensitivity determination of H2O2. Factors and performances such as pH, potential, influencing the designed biosensor, were studied carefully. The amperometric detection of H2O2 was carried out at -300 mV in phosphate buffer solution (PBS) (0.1 M) with pH 6.0. This biosensor showed a fast amperometric response (less then 5 s) to H2O2. The levels of the (Relative standard deviation) RSDs (< 3.5%) for the entire analyses reflected a highly reproducible sensor performance. Using the optimized conditions, the detection limit of the biosensor was 1 x 10(-7) M and linear range was from 5 x 10(-6) to 1.25 x 10(-4) M. In addition, this sensor showed long-term stability and good sensitivity.
Find another biosensor using peroxidase in a 2- enzyme biosensor (for another analyte, not glucose. Describe...
1. design a follow-up experiment to investigate the enzyme peroxidase from plants. 2. investigate the effect of pH on enzyme activity.
2.NSAIDs work by inhibiting which enzyme? A. Peroxidase B. Lipoxygenase C. Cyclooxygenase D. Phospholipase
2. As an alternative, people developed another enzyme mediated assay to measure glucose concentration as below: Hexokinase Glucose + ATP Glucose-6-Phosphate + ADP G6PDH G6P + NAD 6-Phosphogluconate + NADH Based on the information above and what you learned so far, how exactly can you determine the glucose concentration from this method? Hint: spectrophotometrically (10 pts)
2 H 20? Which enzyme catalyzes the reaction: H202 + 2H Peroxidase Superoxide dismutase Catalase Oxidase QUESTION 2 Which of the following is the best definition of fermentation? The production of ethanol from glucose The production of energy by substrate level phosphorylation The reduction of glucose to pyruvic acid The complete catabolism of glucose to CO2 and H2O The oxidation of glucose with organic molecules serving as electron acceptors QUESTION 3 The best definition of osmotic pressure is The active...
How would you describe the polarity of the enzyme glucose-6-phosphatase, knowing it is a transmembrane protein?
2. What does the enzyme mutarotase catalyze? Show by an equation using the glucose isomers. 3. Draw the Haworth structures of the following monosaccharides: D-glucopyranose, L- glucopyranose, D-glucofuranose, D-mannopyranose, D-galactopyranose, D-deoxy- ribofuranose, D-fructofuranose, D-fructopyranose. For the purposes of this problem, ignore the distinction betweena and B.
2. The first step in glycolysis is catalyzed by the enzyme herokinase: glucose + ATP glucose-6-P + ADP This reaction can be thought of as two coupled reactions (P is phosphate): (1) glucose + P + glucose-6-P (2) ATP + ADP + P For the overall reaction, AG" = -16.7 kJ/mol. For the hydrolysis of ATP (equation 2), AG = -310 kJ/mol. All reactions are performed at 37°C. (a) What is AGº for reaction 1? Iglucose - 6-11 if AG...
Describe an example of each type of regulation in the context of glucose metabolism. Factors That Determine Activity of Enzymes: 1) Extracellular signal 2) Transcription of specific genes 3) mRNA degradation 4) mRNA translation on ribosome 5) Protein degradation 6) enzyme sequestered in subcellular organelle 7) Enzyme binds substrate 8) Enzyme binds ligand 9) Enzyme undergoes phosphorylation/dephosphorylation 10) Enzyme combines with regulatory protein
E. coli/brings lactose into its cell by using an enzyme called Lac Permease. Once the lactose is inside, another enzyme called B-galactosidase converts the lactose into galactose and glucose. 1. How is E. coll's way of dealing with lactose similar to how your celis do it? 2. How is it different?
Question 2: You have just discovered a new enzyme that catalyzes the degradation of cellulose to glucose. You hope to commercialize this enzyme ultimately to invent a process for biofuel manufacture. In order to determine how efficient the enzyme is and how fast the enzyme catalyzes the reaction, you have to perform some basic enzyme kinetics. Plot the following enzyme data for the enzyme catalyzed reaction and determine the Km and Vmax. Show your graph with correct units on the...