Gel Electrophoresis Why is it important for the gel to solidify? What determines the rigidity of...
Gel Electrophoresis
- Why is it important for the gel to solidify?
- What determines the rigidity of the gel?
- What is the relevance of the loading dye?
- Is bromophenol blue used to stain DNA?
Homework Answers
Answer 1. Gel electrophoresis is the technique to separate DNA fragments of different size through gel. DNA is negatively charged so they move towards positively charged electrodes through gel. Large particle slow down because of solidity of gel and move slow while small fragment move fast. If gel is not solid measuring the speed is not possible because all the fragment will move at equal speed toward electrode.
Answer 2. Rigidity of gel is determined by viscosity stress.
Answer 3. Because DNA is colorless so adding dyes help to determine the rate of movement.
Answer 4. Yes bromophenol blue used to stain DNA because it has a slight negative charge and will migrate in same direction as DNA, allowing the user to monitor the progress of fragment of DNA.
Add Answer to:
Gel Electrophoresis
Why is it important for the gel to solidify?
What determines the rigidity of...
In order for the DNA samples to TRAVEL across the gel accurately, it is important that...
In order for the DNA samples to TRAVEL across the gel accurately, it is important that (Select all that apply) Check All That Apply the voltage is set correctly on the chamber. the electrophoresis buffer is poured over the agarose gel. the pH of the gel is correct. bromophenol blue dye is used. All of the answer choices are correct.
What is the purpose of gel electrophoresis? How is size related to movement through a gel?...
What is the purpose of gel electrophoresis? How is size related to movement through a gel? What is a DNA ladder? Why is it important in gel electrophoresis and how is it used? (note: a ladder is also called a “standard”) What are two indications one can look for to be certain the gel electrophoresis is occurring? What are two strategies to improve the resolution of DNA bands?
6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a...
6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a 8 uL DNA sample before loading it onto the gel? (1 pt)
1a) A band on an electrophoresis gel corresponds to _____ Choices: - the size standard -...
1a) A band on an electrophoresis gel corresponds to _____ Choices: - the size standard - millions of fragments of the same size - a DNA fragment of a particular size - the blue tracking dye b) What is the function of the size standard? Choices: - it allows the researcher to calculate the unknown sizes of the DNA fragments by comparing then to then known sizes. - it marks the location where the fragments should line up. c) Longer...
NA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the...
NA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed. 1)Gel Electrophoresis Procedure The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells. 2)An electric current is passed through the gel. 3) DNA fragments are treated with a dye. 4)A restriction endonuclease is added to the DNA. 5)Using micropipettes, the DNA samples...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
Stuck answering the rest of these 3. Application of DNA gel electrophoresis. DNA gel electrophoresis is...
Stuck answering the rest of
these
3. Application of DNA gel electrophoresis. DNA gel electrophoresis is commonly used in determining familial relationships among individuals, for ex; to establish paternity of a child. This technique is called DNA fingerprinting. In this technique the DNA of parents and children is roughly chopped up into pieces and resolved on an agarose gel. The DNA ill resolve according to their sizes and create a pattern or a "fingerprint". The fingerprint of the child is...
In the nuclease digestion experiment, the scientists use a technique called gel electrophoresis. While this is...
In the nuclease digestion experiment, the scientists use a technique called gel electrophoresis. While this is related to SDS-PAGE used in the separation of membrane proteins, it is NOT THE SAME thing. From the list below, choose all the ways that gel electrophoresis is done for nuclease digestion is different from the gel electrophoresis in SDS-PAGE. Group of answer choices 1.Used to separate DNA or RNA. 2.Used to separate proteins. 3.Prteins and DNA must be separated prior to running the...
What separation method commonly uses a horizontal gel electrophoresis apparatus? Why?
What separation method commonly uses a horizontal gel electrophoresis apparatus? Why?
In order for the DNA samples to TRAVEL across the gel accurately, it is important that (Select all that apply) Check All That Apply the voltage is set correctly on the chamber. the electrophoresis buffer is poured over the agarose gel. the pH of the gel is correct. bromophenol blue dye is used. All of the answer choices are correct.
6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a 8 uL DNA sample before loading it onto the gel? (1 pt)
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
Stuck answering the rest of
these
3. Application of DNA gel electrophoresis. DNA gel electrophoresis is commonly used in determining familial relationships among individuals, for ex; to establish paternity of a child. This technique is called DNA fingerprinting. In this technique the DNA of parents and children is roughly chopped up into pieces and resolved on an agarose gel. The DNA ill resolve according to their sizes and create a pattern or a "fingerprint". The fingerprint of the child is...
Most questions answered within 3 hours.
-
You are attempting to calculate a firm’s free cash flow to
equity. You know the following...
asked 5 minutes ago -
the following reaction occurs in a balloon containing
N2O2 gas
N2O4(g)=2NO2(g)
will the volume of the...
asked 39 minutes ago -
answer the questions throughout this program
public class Day implements Comparable {
Private Boolean atWork;...
asked 47 minutes ago -
This is C++ code for parking fee management program
#include <iostream>
#include <iomanip>
using namespace std;...
asked 58 minutes ago -
The free energy change for the following reaction at 25 °C, when
[Sn2+] = 1.17 M...
asked 2 hours ago -
An MNE is this kind of industry when competition in one country
is essentially independent of...
asked 4 hours ago -
. For this set of questions, determine what
proportion of a normal distribution is located betweeneach...
asked 4 hours ago -
A college student is employed as a door-to-door newspaper
salesman. Historical data suggests that the student...
asked 5 hours ago -
MATLAB HW 11 problem using Switch Case and Input commands
Write a script file that calculates...
asked 5 hours ago -
Considering gravitational time dilation, calculate the time that
passes in Earth’s surface while 1 hour passes...
asked 6 hours ago -
Minitab Problem: Take the Lake Hume June rainfall data and find
use the processes outlined in...
asked 7 hours ago -
X Company is trying to decide whether to continue using old
equipment to make Product A...
asked 7 hours ago