3. You inoculate 1 liter of medium with an E. coli culture to an initial cell...
3. You inoculate 1 liter of medium with an E. coli
culture to an initial cell density of 104 cells/ml. The culture
grows exponentially with a generation time of 20 minutes. What is
the culture density after 24 hours?
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3. You inoculate 1 liter of medium with an E. coli
culture to an initial cell...
You inoculate a strain of E. coli into a broth culture and allow it to grow....
You inoculate a strain of E. coli into a broth culture and allow it to grow. The broth medium contains three sugars: glucose, lactose, and maltose. The strain you are growing prefers lactose to maltose. You measured the growth by doing a viable cell count and drew a growth curve after calculating the number of CFU/ml over time. There was an initial lag phase, followed by an exponential phase. There was a second lag phase of approximately 30 minutes followed...
You inoculate a strain of E. coli into a broth culture and allow it to grow....
You
inoculate a strain of E. coli into a broth culture and allow it to
grow. The broth medium contains three sugars: glucose, lactose, and
maltose. The strain you are growing prefers lactose to maltose. You
measured the growth by doing a viable cell count and drew a growth
curve after calculating the number of CFU/ml over time. There was
an initial lag phase, followed by an exponential phase. There was a
second lag phase of approximately 30 minutes followed...
Consider a culture of E. coli growing in exponential phase with a generation time of 20...
Consider a culture of E. coli growing in exponential phase with a generation time of 20 minutes. Beginning with 2 x 106 cells per ml, how long will it take the culture to reach a density of about 3.2 x 107 cells per ml?
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect...
An exponentially growing culture has a specific growth rate constant, K = 2.4 day-1. What is...
An exponentially growing culture has a specific growth rate constant, K = 2.4 day-1. What is the generation time? A test tube of fresh medium is inoculated with initial cell population of 6 x 105 cells/mL. Assuming no lag time, how long will it take for the culture to reach 8 x 109 cells/mL.
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass...
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass of E. coli (in pounds) that will accumulate when a single cell of E. coli grows exponentially for 50 hours in these conditions, assuming that nutrients are not limiting. Show your work. (An E. Coli cell has a mass of 10-12gram/cell).
A batch culture of E. coli was initiated with a medium containing 10 g/L of glucose,...
A batch culture of E. coli was initiated with a medium
containing 10 g/L of glucose, 2 g/L of ammonia, and 0.1 g/L of
cells
A batch culture of E. coli was initiated with a medium containing 10 g/L of glucose, 2 g/L of ammonia, and 0.1 g/L of cells. At the end of culture, 0.1 g/L of glucose remained in the medium. A total of 5.1 g/L of E. coli were recovered. The elemental composition of the cells was...
E. coli has a doubling time of 20 minutes, if a piece of meat is contaminated...
E. coli has a doubling time of 20 minutes, if a piece of meat is contaminated with 1 E. coli cell how many cell would it contain after 12 hours? What about 24 hours?
You have a stock of an E. coli cell culture of an unknown concentration. You want...
You have a stock of an E. coli cell culture of an unknown concentration. You want to set up a serial dilution experiment so that you can count the viable colonies and determine the concentration of the stock in cells/ml. In tube A, you add 1 ml of the stock to 999 ml of media. In tube B, you add 1 ml of the material from tube A to 24 ml of media. In tube C, you add 50 microliters...
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How...
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How many CFUs are expected if you plated 0.2 mL of a 104 dilution? (Hint: Consider the average number of cells per CFU) 18. At 65 minutes, the cells were transferred to a new medium in which the growth rate (H) was three times lower. What is the expected number of cells after 60 minutes of growth following the transfer? 19.By what factor did the...
You inoculate a strain of E. coli into a broth culture and allow it to grow. The broth medium contains three sugars: glucose, lactose, and maltose. The strain you are growing prefers lactose to maltose. You measured the growth by doing a viable cell count and drew a growth curve after calculating the number of CFU/ml over time. There was an initial lag phase, followed by an exponential phase. There was a second lag phase of approximately 30 minutes followed...
You
inoculate a strain of E. coli into a broth culture and allow it to
grow. The broth medium contains three sugars: glucose, lactose, and
maltose. The strain you are growing prefers lactose to maltose. You
measured the growth by doing a viable cell count and drew a growth
curve after calculating the number of CFU/ml over time. There was
an initial lag phase, followed by an exponential phase. There was a
second lag phase of approximately 30 minutes followed...
A batch culture of E. coli was initiated with a medium
containing 10 g/L of glucose, 2 g/L of ammonia, and 0.1 g/L of
cells
A batch culture of E. coli was initiated with a medium containing 10 g/L of glucose, 2 g/L of ammonia, and 0.1 g/L of cells. At the end of culture, 0.1 g/L of glucose remained in the medium. A total of 5.1 g/L of E. coli were recovered. The elemental composition of the cells was...
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How many CFUs are expected if you plated 0.2 mL of a 104 dilution? (Hint: Consider the average number of cells per CFU) 18. At 65 minutes, the cells were transferred to a new medium in which the growth rate (H) was three times lower. What is the expected number of cells after 60 minutes of growth following the transfer? 19.By what factor did the...
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