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A colleague presents you the structure of a hexapeptide (sequence: KLVFFA) at neutral pH. With your...
2. (10 points) The structure for the amino acid histidine is shown below (at pH 1)....
2. (10 points) The structure for the amino acid histidine is shown below (at pH 1). Write the chemical equations for the ionic dissociations of histidine (as the pH increases) Determine the isoelectric point for histidine and indicate the structure present at the isoelectric point. The pka values for histidine are 1.82 (-CO2H). 9.17 (NH3'), and 6.00 (R group). OOH "HaN NH "INP
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
Post-Lab Questions 1. Look up the structure in your textbook of the amino acid you titrated....
Post-Lab Questions 1. Look up the structure in your textbook of the amino acid you titrated. Write the structure you expect at pH (a) 2.5 and (b) 12 2. Compare your isoelectric point value (pl value) with the value of the amino acid listed in your textbook. How does it compare? Into which class does your amino acid fall? 3. Which data point can you obtain with greater accuracy from your graph-the pK values from the "legs" or the isoelectric...
Name_Rachel A Date TITILLEELELE PROTEINSI Questions 1. Which amino acids used in this experiment have acidic...
Name_Rachel A Date TITILLEELELE PROTEINSI Questions 1. Which amino acids used in this experiment have acidic R-groups, which have basic R-groups, and which have neutral R-groups? Write acidic", "basic" or "neutral next to each amino acid below. • Glycine: • Glutamic acid: • Arginine: • Tyrosine: 1. Circle all the charged carboxyl groups (carboxylate groups) in the amino acid structures below. | HN-CH-- HN-CH-C-ộ HN-CH-C ó Glycine (Gly) Glutamic acid or glutamate (Glu) C=NH2 NH2 Arginine (Arg) UUUUU Proteins Chemistry...
2. (7 pts) What is the sequence of the following HEXAPEPTIDE? *PROVIDE WORK/ANSWER for B-F! (Use...
2. (7 pts) What is the sequence of the following HEXAPEPTIDE? *PROVIDE WORK/ANSWER for B-F! (Use deductive reasoning) Observations from Sequencing Experiments: A. The Sequence contains the following amino acids: Y, R, M, K, G,D B. Reaction with 1-fluoro-2,4-dinitrobenzene yielded DNP-G. C. Treatment with chymotrypsin yielded two tripeptides. D. Treatment with trypsin yielded three dipeptides. E. Treatment with carboxypeptidase yielded methionine. F. N-terminal sequencing of one of the chymotrypsin-produced tripeptides yielded DNP-K Explanation and summary of protein sequencing techniques •...
The one-letter sequence is: WATER a) Draw the peptide (R-groups trans), indicating charges, in pr...
The one-letter sequence is: WATER
a) Draw the peptide (R-groups trans), indicating charges, in
predominant form found at pH = 0.
b) What is the isoelectric point?
c) What is the average charge on the population of peptide
macromolecules at pH = 2.2?
d) What is the average charge on the population of peptide
macromolecules at pH = 12.5?
TABLE 4.1 Amino Acid Alanine Arginine Asparagine Aspartic acid Cysteine....« Glutamic acid Glutamine Glycine Histidine Isoleucine Leucine Lysine … Methionine Phenylalanine...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pI can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pK values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pI value...
(7 pts) What is the sequence of the following HEXAPEPTIDE? *PROVIDE WORK/ANSWER for B-F! (Use deductive...
(7 pts) What is the sequence of the following HEXAPEPTIDE? *PROVIDE WORK/ANSWER for B-F! (Use deductive reasoning) Observations from Sequencing Experiments: A. The Sequence contains the following amino acids: Y, R, M, K, G, D B. Reaction with 1-fluoro-2,4-dinitrobenzene yielded DNP-G. ___ ___ ___ ____ ___ ___ y = 0.058x + 0.0081 y = 0.2711x + 0.0105 -0.02 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 -0.2 -0.1 0 0.1 0.2 0.3 0.4 0.5 Velocity ( mM/min) -1 [S] mM-1...
Aspartic acid has three pKa values, 2.09, 3.86 and 9.82. a. On the following structure for...
Aspartic acid has three pKa values, 2.09, 3.86 and 9.82. a. On the following structure for this amino acid circle the protons that can be removed and indicate the corresponding pKa value? b. Determine the value of the isoelectric point for this amino acid. c. Sketch the structure of the zwitterion. d. If electrophoresis is carried out at pH is 7.0, will this amino acid migrate to the anode(+) or cathode(-).
2. (10 points) The structure for the amino acid histidine is shown below (at pH 1). Write the chemical equations for the ionic dissociations of histidine (as the pH increases) Determine the isoelectric point for histidine and indicate the structure present at the isoelectric point. The pka values for histidine are 1.82 (-CO2H). 9.17 (NH3'), and 6.00 (R group). OOH "HaN NH "INP
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
Post-Lab Questions 1. Look up the structure in your textbook of the amino acid you titrated. Write the structure you expect at pH (a) 2.5 and (b) 12 2. Compare your isoelectric point value (pl value) with the value of the amino acid listed in your textbook. How does it compare? Into which class does your amino acid fall? 3. Which data point can you obtain with greater accuracy from your graph-the pK values from the "legs" or the isoelectric...
Name_Rachel A Date TITILLEELELE PROTEINSI Questions 1. Which amino acids used in this experiment have acidic R-groups, which have basic R-groups, and which have neutral R-groups? Write acidic", "basic" or "neutral next to each amino acid below. • Glycine: • Glutamic acid: • Arginine: • Tyrosine: 1. Circle all the charged carboxyl groups (carboxylate groups) in the amino acid structures below. | HN-CH-- HN-CH-C-ộ HN-CH-C ó Glycine (Gly) Glutamic acid or glutamate (Glu) C=NH2 NH2 Arginine (Arg) UUUUU Proteins Chemistry...
2. (7 pts) What is the sequence of the following HEXAPEPTIDE? *PROVIDE WORK/ANSWER for B-F! (Use deductive reasoning) Observations from Sequencing Experiments: A. The Sequence contains the following amino acids: Y, R, M, K, G,D B. Reaction with 1-fluoro-2,4-dinitrobenzene yielded DNP-G. C. Treatment with chymotrypsin yielded two tripeptides. D. Treatment with trypsin yielded three dipeptides. E. Treatment with carboxypeptidase yielded methionine. F. N-terminal sequencing of one of the chymotrypsin-produced tripeptides yielded DNP-K Explanation and summary of protein sequencing techniques •...
The one-letter sequence is: WATER
a) Draw the peptide (R-groups trans), indicating charges, in
predominant form found at pH = 0.
b) What is the isoelectric point?
c) What is the average charge on the population of peptide
macromolecules at pH = 2.2?
d) What is the average charge on the population of peptide
macromolecules at pH = 12.5?
TABLE 4.1 Amino Acid Alanine Arginine Asparagine Aspartic acid Cysteine....« Glutamic acid Glutamine Glycine Histidine Isoleucine Leucine Lysine … Methionine Phenylalanine...
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