Question

2. Calculate the final concentration of yeast and buffer in each of the four reaction tubes. Enter your results in Table 1 below. Table 1. Standard Fermentation Reactions 1 pt Reagents Reaction mix (ml) Tube #1 Tube #2 Tube #3 Tube #4 Water 7.0 6.0 4.0 5.0 0.2 M NaP buffer, pH 7 2.0 2.0 2.0 2.0 60% glucose 1.0 1.0 1.0 0 __% yeast 1.0 3.0 3.0 Total volume of each reaction 10.0 10.0 10.0 Final concentration of glucose in reaction Final concentration of yeast in reaction 0% Final concentration of buffer in reaction What are your predictions for the fermentation rate for each of the reaction tubes in Table 1? Explain 1 pt Enter your answer here

Answer 1

Answer:

$\rightarrow$Calculation is based on the volume of glucose / yeast / buffer in relation to 100ml, as this will corresponds to percentage.

$\rightarrow$tube 1

• glucose concentration- 1X 100/ 10 = 10%
• buffer concnentration.2 X100/10= 20%

$\rightarrow$tube 2

• glucose concentration- 1X100/ 10= 10%
• Yeast concentration= 1X 100/ 10= 10%
• buffer concentraiton= 2X 100/10= 20%

$\rightarrow$tube 3

• glucose concentraiton= 1X100/10=10%
• yeast concentration= 3X100/ 10=30%
• buffer cincentration= 2X 100/10= 20%

$\rightarrow$tube 4

• glucose concentration= 0X100/10= 0
• yeast concentration= 3X 100/ 10= 30%
• buffer concentration= 2X 100/10= 20%

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