Homework Answers
In this co-translational translocation of a protein in ER Lumen, the protein is synthesised by membrane bound ribosomes. The protein will move in unfolded protein form. The signal sequence is located at N-terminal and cleavable.The order for the translocation of a soluble protein in ER Lumen will be :
#1. Ribosomes binds mRNA.
#2. Translation begins.
#3. SRP(Signal recognition particle present in cytosol) binds ER signal sequence.
#4. Translation pause (elongation step is paused).
#5. SRP binds SRP receptor (present on ER membrane).
#6. ER signal sequence binds to the translocator channel (gated channel).
#7. Translocation of the protein through the translocator begins (N-terminal enters first with the help of TRAM).
#8. Signal sequence is cleaved by signal peptidase (because signal is non-polar , can't enter ER Lumen, so is cleaved).
#9. Translation of the polypeptide finishes.
#10. Ribosomes dissociates from ER membrane.
Thus, soluble protein is translocated to ER Lumen.
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