Question

Three genes with recessive mutant phenotypes are located on the same chromosome in maize: short stem...

Three genes with recessive mutant phenotypes are located on the same chromosome in maize: short stem (ss), defective endosperm (de), and virescent seedling (v). A plant with all three mutant phenotypes is crossed with a true-breeding, wild-type plant. Resulting F1 plants are then backcrossed to the parental mutant line to produce the following progeny:

Waxy    Shrunken    Virescent   Number

ss                 +                     +                 290

   +                de                    v                 282

ss                de                  +                   89

   +                +                      v                   92

   +                de                    +                 480

ss                 +                     v                 477

   +               +                      +             1482

ss                de                    v               1475

Draw a chromosome map with the correct distances (rounded to the nearest cM) noted between

adjacent pairs of genes.

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Answer #1

To find the gene order, see the parents genotype, whose no. will be always higher then check the genotype of double cross over , whose no. will be always less. After that arrange gene order in parental genotype to get double recombinant.

Gene order = + + +

ss v de

To find distance between 2 consecutive gene, add double cross over recombinants to the no. of single recombinant after single cross over and divide it by total no. of progeny

Distance between gene ss and gene v = 16.13 CM

Distance between gene v and gene de = 24.38 CM

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