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Explain mcrbc enzyme in Arabidopsis thaliana?

Explain mcrbc enzyme in Arabidopsis thaliana?

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McrBC is a restriction endonuclease. It cleaves the DNA containing methyl cytosine. Arabidopsis thaliana has retained the multi layered methylation system. This system contributes gene and transposon silencing, imprinting and genome stability.
Methylation of cytosine at five carbon position makes it 5-methyl cytosine in most of the plants. Transcription factors are blocked from binding to the transcription machinery and transcription is prevented or gene silencing is caused.
The mcrbc enzyme cleaves the methylated DNA. It belongs to the AAA+ family of proteins. There are two proteins that are expressed from mcr b gene. They are one short protein and another one long protein. Only the long form of protein binds to DZNA at methylated sites, the short form of DNA will be present away from the DNA.

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