Explain mcrbc enzyme in Arabidopsis thaliana?
McrBC is a restriction endonuclease. It cleaves the DNA
containing methyl cytosine. Arabidopsis thaliana has retained the
multi layered methylation system. This system contributes gene and
transposon silencing, imprinting and genome stability.
Methylation of cytosine at five carbon position makes it 5-methyl
cytosine in most of the plants. Transcription factors are blocked
from binding to the transcription machinery and transcription is
prevented or gene silencing is caused.
The mcrbc enzyme cleaves the methylated DNA. It belongs to the AAA+
family of proteins. There are two proteins that are expressed from
mcr b gene. They are one short protein and another one long
protein. Only the long form of protein binds to DZNA at methylated
sites, the short form of DNA will be present away from the DNA.
what is the relevance of saccharomyces cerevisiae and Arabidopsis thaliana in biotechnology experiments?
can someone label the yellow part at the tip? this a Arabidopsis thaliana seed
1. Arabidopsis thaliana was the first plant to have its genome sequenced and is an important scientific model organism. It is a flowering plant and its genome is only 157 million base pairs. The flowering plant Paris japonica, in contrast, has a genome that is 150 billion base pairs in length. Does this mean that P. japonica is a more complex and evolutionary successful organism? Explain your answer.
Arabidopsis thaliana is a plant model species with a somatic chromosome number of 2n=10 Suppose each chromosome contains 10^6 bp of DNA, how long will it take DNA polymerase to replicate the entire genome from 10 origins of replication in each chromosome at a rate of 1000 bp per second per replication fork?
You are currently studying Arabidopsis thaliana (a small flowering plant) and discover a gene that controls when the plant flowers. You would like to know if the gene is present in Oryza sativa (rice). You do not have the physical plant or genomic DNA, and the sequence of the rice genome is incomplete. You do have an Oryza sativa cDNA library. How might you identify if Oryza sativa likely has the gene.
3. Belgian scientists discovered that certain mutations in common wall cress (Arabidopsis thaliana) cause excess auxin production. Predict the impact on the plant’s phenotype. 4. Cattle in industrial dairy farms are typically given rBST, or recombinant bovine somatotropin. This animal hormone increases a cow’s milk production, but there is concern that such hormones may have unforeseen effects on milk-drinking humans. There is no similar concern about plant hormones in the plant foods we eat. Why not?
(4 points) In Arabidopsis thaliana, two genes, HOT2 and STO, are involved in heat and salt tolerance, respectively. You want to make a plant that is resistant to both stresses. As a first step in the generation of this plant, you cross a true breeding plant with a mutation in HOT2 that causes increased heat tolerance with a true breeding plant that has a mutation in STO that causes increased salt tolerance. This cross produces Fi double heterozygotes that do...
Arabidopsis thaliana, a plant, has small leaf projections called trichomes. A search for mutants revealed two mutant strains, A and B, both of which lack trichomes. Strain A plant was propagated until it was completely true-breeding and then crossed to a true-breeding wild-type plant. The F1 was all wild-type. The F2 results are below. The plant geneticist was unable to form a true-breeding plant from strain B plant. When a strain B mutant was crossed to wild-type, the F1 data...
Genetics: What is the expected phenotype of the following mutants, either inDrosophila melanogaster or in Arabidopsis thaliana? (i) Overexpression of both bicoid and nanos in a female fly. (ii) Overexpression of the homeotic gene, antennapedia, in a female fly lacking hunchback. (iii) Deletion of the pair-rule gene, even-skipped, in a male fly overexpressingnanos. (iv) Overexpression of the Class A gene, APETALA1, in a plant lacking the Class B gene, APETALA3. (v) Overexpression of the Class C gene, AGAMOUS, in a...
I have a strain of Arabidopsis with a mutation in a gene that encodes an enzyme that removes methyl groups from cell wall polysaccharides (it is called a methylesterase). I extracted the cell walls of this strain to get three fractions. The first fraction was obtained by extracting with steam and hot water, the second fraction was obtained by extracting with 2 M NaOH, and the final fraction was the residual, insoluble cell wall fraction. Name the type of cell...