Suppose you used CRISPR/Cas9 to target a gene without providing a repair template, and that this...
Suppose you used CRISPR/Cas9 to target a gene without providing a repair template, and that this experiment results in a deletion of one DNA base pair right after the ATG, of the gene you are studying. How might this affect protein function?
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Suppose you used CRISPR/Cas9 to target a gene without providing
a repair template, and that this...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
the following scenarios require application of CRISPR-cas9 technology. Identify which technologies are the best fit for...
the following scenarios require application of CRISPR-cas9
technology. Identify which technologies are the best fit for each
research scenario
Cas9 and HDR 1. Researchers want to make an endonuclease that binds to target DNA, but does not cut the DNA. This will allow them to target specific areas of the genome for other modifications without making cuts. dCas9 dCas9 and repression domain 2. Researchers are studying a proposed oncogene in mice. They want to examine what happens when the gene...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is most correct? Capping the sgRNA is essential for its stability. The Cas9 mRNA is is used to target the native Cas9 protein to the locus to be mutated. the sgRNA uses DICER to cut up a the twenty nucleotide target region of the DNA, starting at the 3-prime end. O neomycin is used to select the cells that inherit the Cas9 insertion. O the...
Funcions of the After Cas9 cleaves the DNA, cellular enzymes will attempt to repair the break....
Funcions of the After Cas9 cleaves the DNA, cellular enzymes will attempt to repair the break. Most of the time, these enzymes repair the DNA without errors. However, Cas9 will keep cutting the DNA at the same location until an error is made. 7. DNA repair errors include losing or inserting random nucleotides at the cut site. Explain how these changes might inactivate a gene. PART 2: Inactivating Genes in Butterflies Robert Reed, a biologist at Cornell University, wanted to...
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
4. What is the function of each component of the CRISPR-Cas9 system? (1.5 pt) PAM site:...
4. What is the function of each component of the CRISPR-Cas9 system? (1.5 pt) PAM site: Guide RNA: Cas9: 5a. In the DNA below, the "I" line indicates where there is a mutation in a gene. Design a gRNA to find this mutation. Your gRNA will be 8 bases long and will not include the PAM sequence. Keep the following in mind: [2 pts total] i. Scan the top strand left to right and find the PAM site. ii. When...
please help answer these questions 3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
A number of advances have been made in biotechnology. CRISPR/Cas9 one of the most controversial, and...
A number of advances have been made in biotechnology.
CRISPR/Cas9 one of the most controversial, and is getting a lot of
current media attention. It is a method by which scientists can
precisely edit DNA sequences at exact locations. Benefits obviously
include the potential to “repair” mutated genes that cause disease.
In fact, preliminary results from one of the earliest clinical
trials of CRISPR/Cas9 provide evidence that the technique is safe
and feasible to use for treating human diseases. What...
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1....
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1. Match the CRISPR component with its function. (matching the upper case letters to the lower case letters) A). Cas9 B). gRNA (sometimes called sgRNA) C). Non-homologous End Joining (NHEJ) D). Homology-directed Repair (HDR) a). A short RNA molecule that guides a DNA cutting enzyme to a specific location in genomic DNA. b). A type of DNA repair mediated by a set of enzymes all...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do bacteria use CRISPR? 4.How is the enzyme targeted to a specific DNA sequence? 5.what need to be done in order to make sure the proper human protein is made in bacteria cells? 6.How can you check for proper orientation of the inserted DNA in the plasmid? 7. Why are iPSCs useful for studying hereditary neuronal diseases such as Alzheimer's. 8. How are pig cells...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
the following scenarios require application of CRISPR-cas9
technology. Identify which technologies are the best fit for each
research scenario
Cas9 and HDR 1. Researchers want to make an endonuclease that binds to target DNA, but does not cut the DNA. This will allow them to target specific areas of the genome for other modifications without making cuts. dCas9 dCas9 and repression domain 2. Researchers are studying a proposed oncogene in mice. They want to examine what happens when the gene...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is most correct? Capping the sgRNA is essential for its stability. The Cas9 mRNA is is used to target the native Cas9 protein to the locus to be mutated. the sgRNA uses DICER to cut up a the twenty nucleotide target region of the DNA, starting at the 3-prime end. O neomycin is used to select the cells that inherit the Cas9 insertion. O the...
Funcions of the After Cas9 cleaves the DNA, cellular enzymes will attempt to repair the break. Most of the time, these enzymes repair the DNA without errors. However, Cas9 will keep cutting the DNA at the same location until an error is made. 7. DNA repair errors include losing or inserting random nucleotides at the cut site. Explain how these changes might inactivate a gene. PART 2: Inactivating Genes in Butterflies Robert Reed, a biologist at Cornell University, wanted to...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
4. What is the function of each component of the CRISPR-Cas9 system? (1.5 pt) PAM site: Guide RNA: Cas9: 5a. In the DNA below, the "I" line indicates where there is a mutation in a gene. Design a gRNA to find this mutation. Your gRNA will be 8 bases long and will not include the PAM sequence. Keep the following in mind: [2 pts total] i. Scan the top strand left to right and find the PAM site. ii. When...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
A number of advances have been made in biotechnology.
CRISPR/Cas9 one of the most controversial, and is getting a lot of
current media attention. It is a method by which scientists can
precisely edit DNA sequences at exact locations. Benefits obviously
include the potential to “repair” mutated genes that cause disease.
In fact, preliminary results from one of the earliest clinical
trials of CRISPR/Cas9 provide evidence that the technique is safe
and feasible to use for treating human diseases. What...
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