Question

1. EORA BACTERIAL GENE, writing left-to-right, show the relative positions of each of these genetic elements as they would appear in the DNA from upstream on the left to downstream on the right. Of course, some of these genetic elements will only be active once they are in mRNA (e.g., the translational start codon), but they are still found encoded in the sequence of DNA onthe left the translational start colon . but they are s RBS ribosome binding site TSS ranscriptional start site I1 AUG translational start codon (AUG) S-L transcriptional stop site (hairpin) TATA TATA box UAA translational stop site (UAA) 35 35 sequence 10 10 sequence polyA polyA addition site CGU an Arginine codon (CGU) first-intron Your answer should look like the following, though this answer is wrong RBS TSS TATA UG SL UAA 35 polyA CGU

Answer 1

DNA 5’            -35       RBS     -10       TATA S-L UAA Poly A CGU TSS AUG 3’

DNA 3’ TGC ACG TGC ATG GAT CCG CGC AAT ATA TAC GCT ATA TCG ATC TAC GAC GAT CGA CTA ATA TAT CGA CTA GCG AAC ACT ACC 5’

mRNA5’ ACG UGC ACG UAC CUA GGC GCG UUA UAU AUG CGA UAU AGC UAG AUG CUC GUA GCU GAU UAU AUA GCU GAU CGC UUG UGA UGG 3’

Protein Seq      5’ -thr- cys-thr-tyr-leu-gly-ala-leu-tyr-met-arg-tyr-ser-Stop-3’