you are testing the growth of a microbe in a particular food stuff.based on comparison with...
you are testing the growth of a microbe in a particular food stuff.based on comparison with turbidity standards you have a cell prep of 10^8 per ml.you need a starting concentration of 10 ^6 cells per ml.devise the shortest dilution scheme that will give you 10^6 cells per ml.
Homework Answers
The shortest dilution scheme that can be used is serial
dilution.
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you are testing the growth of a microbe in a particular food
stuff.based on comparison with...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
this is a bacterial growth curve experiment . please explain rhe results Procedure: You will follow...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting...
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting with tube 1 and ending in tube 9 with an initial concentration of 100 ug/mL tube # 1 2 3 4 5 6 7 8 9 10 [control] Kanamycin concentration (ug/ml) 100 ? ? ? ? ? ? ? ? 0 growth no no no no no yes yes yes yes yes #2) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw...
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4 cells/mL and need to produce a solution of 56 cells/mL set up detailed serial dilution given 8mL of 4.1 x10^3 cells/ML and need approximitly 100 cells/mL. set up detailed serial dilution by determining the aliquot to be transfered (v1) from the solution to get 100 cells/mL in a final volume of 10 mL given 10mL cell suspension of NIH cells at 3.8 x10^5 cells/ML...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
This viral growth curve was generated as follows: 1.0 ml of a 10-5 dilution of a...
This viral growth curve was generated as follows: 1.0 ml of a 10-5 dilution of a viral preparation was added to 5 X 10^8 cells at time 0 (final volume: 1.0 ml). A plaque assay done with 0.1 ml of the 10-5 dilution of the original viral preparation gave 100 PFU. 11.What was the M.O.I at point A? 12.If the burst size was 100, what was the viral concentration at point B? 13.How many additional infectious cycle can be completed...
You want to dilute and plate an E. coli culture that has 3 *10 8 cells/ml....
You want to dilute and plate an E. coli culture that has 3 *10 8 cells/ml. If you needed to dilute the above culture, starting from the undiluted stock, to a concentration of 500 cells/ml, how could you perform a serial dilution to achieve the desired final concentration?
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
What is the concentration of cells in the starting sample if 1 ml was plated per...
What is the concentration of cells in the starting sample if 1
ml was plated per dilution and the starting sample was river
water?
Report your answer below as a decimal with one significant digit
after the decimal and report the exponent the separate box. See
this example, if your answer is 5.2 x 108 you would
report it as "5.2"
You have the following results from a serial dilution experiment: Sample Dilution Plate Count 1:101 Too many to count...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
What is the concentration of cells in the starting sample if 1
ml was plated per dilution and the starting sample was river
water?
Report your answer below as a decimal with one significant digit
after the decimal and report the exponent the separate box. See
this example, if your answer is 5.2 x 108 you would
report it as "5.2"
You have the following results from a serial dilution experiment: Sample Dilution Plate Count 1:101 Too many to count...
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