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biochemistry calculate enzyme activity
enzyme activily Calucabion 0.0157 min/ mH 0.2mL unit should be
Help! Calculate the concentration of lysozyme activity if 150uL of enzyme is added to 850uL of...
Help! Calculate the concentration of lysozyme activity if 150uL of enzyme is added to 850uL of substrate to yield a) -0.0435abs/min at A450? b) 0.000abs/min at A450? The blank used was water...abs/min=0.000. df is 20
You have a 25 ml enzyme sample that has a total of 100 micro-moles/min of activity...
You have a 25 ml enzyme sample that has a total of 100 micro-moles/min of activity If you were to assay the enzyme, how much of it would you need in your assay in order to get an absorbance of 0.50 in a 30-minute assay. The product E=13,800M-1cm-1 and you are using a standard 1mL cuvette Beer-lambert law: A=CIE I=1
You have a 25 ml enzyme sample that has a total of 100 micro-moles/min of activity If you were to...
You have a 25 ml enzyme sample that has a total of 100 micro-moles/min of activity If you were to assay the enzyme, how much of it would you need in your assay in order to get an absorbance of 0.50 in a 30-minute assay. The product E=13,800M-1cm-1 and you are using a standard 1mL cuvette Beer-lambert law: A=CIE I=1
[Alcohol] mMAD Activity AD Activity AD Activity mM/min Agent A Agent B mM/minmM/min 15.0 2.5 5.0 7.5 45.0 20.0 0.5...
[Alcohol] mMAD Activity AD Activity AD Activity mM/min Agent A Agent B mM/minmM/min 15.0 2.5 5.0 7.5 45.0 20.0 0.5 63.0 10.0 32.0 71.0 2.0 11.7 48.0 4.0 79.0 13.4 66.0 8.0 14.3 83.0 84.0 32.0 90.0 15.5 90.0 1. Using the data provided draw a Michaelis-Menten and Lineweaver-Burk Plot for each of the enzyme activities? When you produce the 2 graphs make sure you have plotted 3 curves for the Michaelis-Menten plot on one graph and the three lines...
If your specific activity of your enzyme is 20 micromoles of product/(min*mg protein), how many micromols...
If your specific activity of your enzyme is 20 micromoles of product/(min*mg protein), how many micromols of product can be attained in 2 minutes given 2 mg of protein? a. 5 mol b. 20 mol c. 80 mol d. cannot be determined from the data provided in the problem
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vma...
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vmax and the amount of enzyme present in this experiment. Use excel to obtain the lineweaver burk plot. Substrate concentration (MM) 1 Initial velocity (umol/min) 167 250 334 376 498 499 100 1,000
Calculate the specific activity for each sample using the formula: 1 unit (specific activity) = 1000...
Calculate the specific activity for each sample using the
formula:
1 unit (specific activity) = 1000 x (A420– (1.75 x
A550) / (T x V x A600)
where:
A420= absorbance of the yellow ONP
A550= cell debris light scattering
T = reaction time in minutes
V = volume of culture assayed (ml)
A600= cell density
Sample
Calculation
Activity
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β galactosidase Activity (10 points) RESULS Ex. 1 1 a: Acoo |...
Consider the enzyme-catalyzed reaction with Vmax=164 (μmol/L)min−1 and KM=32μmol/L. Part A If the total enzyme concentration...
Consider the enzyme-catalyzed reaction with Vmax=164 (μmol/L)min−1 and KM=32μmol/L. Part A If the total enzyme concentration was 6 nmol/L, how many molecules of substrate can a molecule of enzyme process in each minute? Express your answer to three significant figures. kcat kcat = 2.73×104 min−1 Part B Calculate kcat/KM for the enzyme reaction.
Please completely answer the below biochemistry question based on the provided Figure. Please show/explain all work....
Please completely answer the below biochemistry question based
on the provided Figure. Please show/explain all work.
3. The authors of this study studied the ability of N-1,5-dimethylhexylformamide to inhibit mouse ADH1. The activity of the enzyme was measured in the absence of inhibitor, and in the presence of 1.0 μM inhibitor. The data are presented in Table 13.1. Table 13.1: Inhibition of mouse ADH1 by N,1-5-dimethylhexylformamide ADHI velocity, Δ NADH | ADHI velocity, Δ NADH absorbance/min (with inhibitor) Ethanol Concentration,...
Does anyone know how to do this? Thanks in advance! Enzyme Activity (ml) Effect of pH...
Does anyone know how to do this? Thanks in advance!
Enzyme Activity (ml) Effect of pH on (amylase) enzyme activity. For determination of optimum assay pH of the enzyme reaction, 0.05 M Na2HPO4-NaH2PO4 buffer was used. The reaction was carried out for 10 min at 50°C in a shaking water. The enzyme activity was measured and the results are presented on graph, Bars represent means standard deviations for three replicates. 1. What do the error bars in this graph represent?...
[Alcohol] mMAD Activity AD Activity AD Activity mM/min Agent A Agent B mM/minmM/min 15.0 2.5 5.0 7.5 45.0 20.0 0.5 63.0 10.0 32.0 71.0 2.0 11.7 48.0 4.0 79.0 13.4 66.0 8.0 14.3 83.0 84.0 32.0 90.0 15.5 90.0 1. Using the data provided draw a Michaelis-Menten and Lineweaver-Burk Plot for each of the enzyme activities? When you produce the 2 graphs make sure you have plotted 3 curves for the Michaelis-Menten plot on one graph and the three lines...
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vmax and the amount of enzyme present in this experiment. Use excel to obtain the lineweaver burk plot. Substrate concentration (MM) 1 Initial velocity (umol/min) 167 250 334 376 498 499 100 1,000
Calculate the specific activity for each sample using the
formula:
1 unit (specific activity) = 1000 x (A420– (1.75 x
A550) / (T x V x A600)
where:
A420= absorbance of the yellow ONP
A550= cell debris light scattering
T = reaction time in minutes
V = volume of culture assayed (ml)
A600= cell density
Sample
Calculation
Activity
1
2
3
4
5
6
7
8
9
10
β galactosidase Activity (10 points) RESULS Ex. 1 1 a: Acoo |...
Please completely answer the below biochemistry question based
on the provided Figure. Please show/explain all work.
3. The authors of this study studied the ability of N-1,5-dimethylhexylformamide to inhibit mouse ADH1. The activity of the enzyme was measured in the absence of inhibitor, and in the presence of 1.0 μM inhibitor. The data are presented in Table 13.1. Table 13.1: Inhibition of mouse ADH1 by N,1-5-dimethylhexylformamide ADHI velocity, Δ NADH | ADHI velocity, Δ NADH absorbance/min (with inhibitor) Ethanol Concentration,...
Does anyone know how to do this? Thanks in advance!
Enzyme Activity (ml) Effect of pH on (amylase) enzyme activity. For determination of optimum assay pH of the enzyme reaction, 0.05 M Na2HPO4-NaH2PO4 buffer was used. The reaction was carried out for 10 min at 50°C in a shaking water. The enzyme activity was measured and the results are presented on graph, Bars represent means standard deviations for three replicates. 1. What do the error bars in this graph represent?...
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