Purification of Plasmid DNA
Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?
a) Cell lysis
b) Removal of proteins
c) Removal of chromosomal DNA
d) Dissolving plasmid in water
Homework Answers
Answer: d
Explanation: There are some basic steps which are included in obtaining plasmid DNA from E. coli. Firstly, the cell is lysed, further removal of proteins and chromosomal DNA is done. A plasmid is obtained and collected but not in water. Also, further purification is done if necessary.
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
1. What are some reasons for isolating plasmid DNA? 2. What are the functions of sodium...
1. What are some reasons for isolating plasmid DNA? 2. What are the functions of sodium hydroxide and SDS in the cell lysis solution? What is the function of the potassium acetate solution? 3. What structural property of plasmid DNA allows it to be separated from chromosomal DNA during alkaline cell lysis? Was more than one band observed in your plasmid sample after electrophoresis and staining?
recombinant DNA experiment Select the correct words from the drop-down menus for all 4 options (...
recombinant DNA experiment Select the correct words from the drop-down menus for all 4 options ( 1 mark ) Q1) After adding the cell lysis buffer and incubating, neutralisation enables the separation of plasmid DNA from chromosomal DNA based on their different sizes. On neutralisation, the single–stranded DNA species of both chromosomal and plasmid origin (previously separated or denatured by alkali treatment) tend to re-anneal or ‘renature’, but… (i) The Choose. (plasmid / bacterial chromosomal ) DNA can re-anneal faithfully...
2- Which of the following is the first step in DNA isolation from cells in a...
2- Which of the following is the first step in DNA isolation from cells in a clinical sample? a. DNA precipitation b. Precipitation of proteins c. Lysis of cells d. Eluting DNA from a spin column 3- In organic DNA extraction methods, the DNA is found in which fraction? a. Upper aqueous b. Lower aqueous c. Upper hydrophobic d. Lower hydrophobic 4-DNA, after separation from other cellular constituents, is precipitated in which of the following? a. Chloroform b. Isopropanol c....
A polylinker increases the versatility of a DNA plasmid because it: (select all correct answers) A)...
A polylinker increases the versatility of a DNA plasmid because it: (select all correct answers) A) facilitates the cloning of DNA segments. B) contains a DNA sequence that confers resistance to ampicillin. C) contains a DNA sequence to allow the vector to replicate. D)contains DNA recognition sequences for several different restriction enzymes. 2. You have an E. coli plasmid whose sequence contains three EcoRI restriction sites. How many DNA fragments would result from a complete EcoRI restriction enzyme digest? a....
You add (1.04x10^0) ng of plasmid DNA in 50 µL to 100 µL of competent E....
You add (1.04x10^0) ng of plasmid DNA in 50 µL to 100 µL of competent E. coli cells and incubate the mixture on ice for 20 min. Following a heat-shock treatment, the cells are incubated at 37 C for 30 min. At the end of the incubation you spread (1.2000x10^2) µL of the transformation mix on an agar plate and incubate the plate overnight at 37 C. The next morning you observe (6.8400x10^2) colonies on the plate, each colony having...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
llAT&T ? 9:54 PM 124:00:57 Exit 1 1) Many different methods and technologies are available for...
llAT&T ? 9:54 PM 124:00:57 Exit 1 1) Many different methods and technologies are available for the isolation of genomic DNA. All methods involve except which one below? A) Disruption and lyses of the starting material; treatments with proteinase and RNAse B) Separate DNA From Crude Lysate by organic extraction or salting out (via protein precipitation by salt) C) Removal of proteins and other contaminants D) DNA precipitation via ethanol or isopropanol, and DNA recovery E) Run through HPLC gel...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid"...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
1. What are some reasons for isolating plasmid DNA? 2. What are the functions of sodium hydroxide and SDS in the cell lysis solution? What is the function of the potassium acetate solution? 3. What structural property of plasmid DNA allows it to be separated from chromosomal DNA during alkaline cell lysis? Was more than one band observed in your plasmid sample after electrophoresis and staining?
2- Which of the following is the first step in DNA isolation from cells in a clinical sample? a. DNA precipitation b. Precipitation of proteins c. Lysis of cells d. Eluting DNA from a spin column 3- In organic DNA extraction methods, the DNA is found in which fraction? a. Upper aqueous b. Lower aqueous c. Upper hydrophobic d. Lower hydrophobic 4-DNA, after separation from other cellular constituents, is precipitated in which of the following? a. Chloroform b. Isopropanol c....
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
llAT&T ? 9:54 PM 124:00:57 Exit 1 1) Many different methods and technologies are available for the isolation of genomic DNA. All methods involve except which one below? A) Disruption and lyses of the starting material; treatments with proteinase and RNAse B) Separate DNA From Crude Lysate by organic extraction or salting out (via protein precipitation by salt) C) Removal of proteins and other contaminants D) DNA precipitation via ethanol or isopropanol, and DNA recovery E) Run through HPLC gel...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
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