Cloning using a Simple Plasmid and Alpha Complementation
Plasmids are used for carrying out the cloning procedure. Which of the statement is true for plasmids?
a) Bacterial plasmids are linear in nature
b) They are single stranded
c) Insertion of DNA into plasmid allows it to be propagated in host cells and they are known as vectors because of their this property
d) They are not capable of replication in bacteria
Homework Answers
Answer: c
Explanation: Plasmids are double stranded and they are circular in nature. They are capable of replication in bacteria. Insertion of DNA into plasmid allows it to be propagated in host cells and the molecules which are used for propagation by this method are called as vectors.
Cloning using a Simple Plasmid and Alpha Complementation
Which of the following characteristic is not present in a plasmid on a general basis?a) Multiple cloning site (MCS)b) Origin of replication (ori)c) Antibiotic resistance gened) Beta galactose genes
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation....
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation. B. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector. C. To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes D. Transformed bacteria are plated onto media containing the appropriate antibiotic and only bacteria containing the plasmid with antibiotic resistance will grow.
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
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QUESTION 12 The first step in the infection cycle of a virus is replication of its...
QUESTION 12 The first step in the infection cycle of a virus is replication of its genome degradation of the host genome lysis of the host cell attachment to the host cell surface QUESTION 13 Which of the following is NOT a true statement about regulons? Regulons are known to be under positive and negative control Regulons are only known to be under positive control. A regulon describes two or more operons that are under the control of the same...
forme este New York State Depart Request for COMID-19 * Take Test LECTURE EX G Which...
forme este New York State Depart Request for COMID-19 * Take Test LECTURE EX G Which b bhosted.cuny.edu/webapps/assessment/take launch ofcourse assessment id. 1399019 1course de 16 € > C Remaining Time 39 minutes, 18 seconds Question Completion Status QUESTION 39 Two human diseases caused by viruses are O polio and bovine spongiform encephalopathy smallpox and polio O malaria and tuberculosis smallpox and bovine spongiform encephalopathy QUESTION 40 A process that requires a virus to transfer DNA between two bacterial cells...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
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Instructions: Read each question carefully before selecting the one alternative that best completes the statement or answers the question in each case and then indicate it on the answer card. Work at a steady pace and you should have ample time to finish the examination. MULTIPLE CHOICE (2 points each) chi Which group of organisms other than viruses is represented by the most number of individuals on earth? Insects b. Bacteria Archea d. Rats e. Earthworms c. A quaint bacteria...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
QUESTION 12 The first step in the infection cycle of a virus is replication of its genome degradation of the host genome lysis of the host cell attachment to the host cell surface QUESTION 13 Which of the following is NOT a true statement about regulons? Regulons are known to be under positive and negative control Regulons are only known to be under positive control. A regulon describes two or more operons that are under the control of the same...
forme este New York State Depart Request for COMID-19 * Take Test LECTURE EX G Which b bhosted.cuny.edu/webapps/assessment/take launch ofcourse assessment id. 1399019 1course de 16 € > C Remaining Time 39 minutes, 18 seconds Question Completion Status QUESTION 39 Two human diseases caused by viruses are O polio and bovine spongiform encephalopathy smallpox and polio O malaria and tuberculosis smallpox and bovine spongiform encephalopathy QUESTION 40 A process that requires a virus to transfer DNA between two bacterial cells...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
Instructions: Read each question carefully before selecting the one alternative that best completes the statement or answers the question in each case and then indicate it on the answer card. Work at a steady pace and you should have ample time to finish the examination. MULTIPLE CHOICE (2 points each) chi Which group of organisms other than viruses is represented by the most number of individuals on earth? Insects b. Bacteria Archea d. Rats e. Earthworms c. A quaint bacteria...
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