Question

Which of the following forms of mutagenesis is not appropriate for a forward genetics experiment?

a) Ionizing radiation such as X-rays

b) Creating a chimeric reporter gene to determine where in the organism a candidate regulatory sequence drives expression.

c) Using transposons

d) Chemical EMS mutagenesis

In the course of evolution, the most common source of new genes in eukaryotes has been?

a) Robertsonian translocation

b) DNA duplication

c) Alternative splicing

d) Reverse translation

e) Whole genome duplication

Genes that have a common ancestor and have split due to speciation event are known as

a) Homologues

b) Orthologues

c) Paralogues

d) Monologues

Luria and Delbruck’s experiment showed that mutations are directed.

a) True

b) False

An indel in a protein-coding region may result in a frameshift mutation.

a) True

b) False

Mutations occurring within introns will not affect the structure of the protein.

a) True

b) False

F+, F’, and Hfr bacteria can only conjugate with F- bacteria.

a) True

b) False

Transduction alters a bacterial genotype only if homologous recombination occurs.

a) True

b) False

Only eukaryotes have operons.

a) True

b) False

Histone modifications affect chromatin structure and can be epigenetically inherited.

a) True

b) False

While most regulation of gene expression occurs through the control of translation, RNA silencing regulates expression during transcription.

a) True

b) False

Answer 1

1). b) Creating a chimeric reporter gene to determine where in the organism a candidate regulatory sequence drives expression.

The determination of genetic poof or genetic basis that causes a specific phenotypic change is known as the forward genetics. In this, the mutations are induced by using mutagens such as the radiation, chemicals, or transposable elements. The mutant genes are isolate for the gene mapping. In reverse genetics, the phenotypes are studied to determine the effects of altered gene sequences, they by the function of the gene is assessed.