You are developing a purification scheme for succinyl-CoA synthetase and want to use affinity chromatography in...
You are developing a purification scheme for succinyl-CoA synthetase and want to use affinity chromatography in your purification. Which affinity resin will be most useful in your purification of succinyl-CoA synthetase?
Group of answer choices
Chromatography resin with bound citrate
Chromatography resin with bound GDP
Chromatography resin with bound NADH
Chromatography resin with bound fumarate
Chromatography resin with bound succinate
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You are developing a purification scheme for
succinyl-CoA synthetase and want to use affinity chromatography in...
acetyl-CoA COA The reactions of the citric acid cycle are shown in the image. As labeled...
acetyl-CoA COA The reactions of the citric acid cycle are shown in the image. As labeled in the diagram, reactions 1, 3, and 4 are regulation points in the citric acid cycle. oxaloacetate citrate synthase citrate NADH +Hi 7 malate dehydrogenase NAD malate aconitase 2 Which molecule inhibits reaction 1? isocitrate 7 fumarase NAD isocitrate dehydrogenase 3 NADH + H+ O citrate O NAD acetyl-CoA + CO2 fumarate C-ketoglutarate FADH succinate O dehydrogenase FAD O-ketoglutarate a dehydrogenase NAD + COA...
Part B Use the structures given in the cycle shown before this part identify the enzymes involved in each step of the citric acid cycle by completing each sentence Match the words in the left column...
Part B Use the structures given in the cycle shown before this part identify the enzymes involved in each step of the citric acid cycle by completing each sentence Match the words in the left column to the appropriate blanks in the sentences on the right. View Available Hint(s) Reset Help isocitrate dehydrogenase aconitase citrate synthase fumarate hydratase succinate dehydrogenase succinyl-CoA synthetase a-ketoglutarate dehydrogenase malate dehydrogenase In step 1, oxaloacetate condenses with acetyl-CoA to form citrate, which is catalyzed by...
12. Which enzyme catalyzes the reaction shown? Oo 9% a. succinyl-CoA synthetase b. succinate dehydrogenase c....
12. Which enzyme catalyzes the reaction shown? Oo 9% a. succinyl-CoA synthetase b. succinate dehydrogenase c. pyruvate dehydrogenase d、a-ketoglutarate dehydrogenase 13. Which of the reactions of the citric acid cycle requires FAD as a coenzyme? a. the conversion of isocitrate to a-ketoglutarate b. the conversion of citrate to isocitrate c. the conversion of succinate to fumarate d. the conversion of malate to oxaloacetate 14. Another name for Complex I in the electron transport chain is a. cytochrome c oxidase. b....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
Can someone quick answer/check these questions? Thank you, will rate! (the answers that I have came...
Can someone quick answer/check these questions? Thank you, will rate! (the answers that I have came up with are in bold) Please help 51- Glycogenesis involves the following steps a) isomerisation b) activation c) elongation or coupling d) branching e) all of the above 52 During the breakdown of glycogen, free glucose is formed from A. the reducing end. B. the non-reducing end. C. glucose residues in an a 1-->4 linkage to the chain. D. glucose residues in an...
The common molecule produced from all foods at the second stage of catabolism is A) ADP. B acetyl...
just checking my answers, except for number 10.
The common molecule produced from all foods at the second stage of catabolism is A) ADP. B acetyl-CoA. C) glucose. D) citric acid. Which group of compounds includes only low-energy molecules? 1. 2. Hih A)ATP; acetyl-CoA; NADH; FADH2 C) ADP: CO2; NADH; FADH2 DONO BADP: acetyl-CoA; NAD+:FAD-mau ADP: CO2: NAD+, FAD After O2 has been reduced in the electron transport chain, the oxygen atoms are part of what molecules? A) ATP 3....
Question 3. Draw the tripeptide: Val-Ser-Ala. Include a phosphoryl group on the amino acid sidechain that...
Question 3. Draw the tripeptide: Val-Ser-Ala. Include a phosphoryl group on the amino acid sidechain that is most likely to undergo phosphorylation. Question 4. A protein retained on an ion exchange chromatography column is usually eluted off the column by (circle the correct answer) A. increasing the salt concentration in the buffer B. adding the protein's free ligand. C. changing the temperature of the elution buffer. D. allowing the retained protein to naturally come off the column after the non-specifically...
Please answer both of the questions. Please write neatly and also make sure you answer is...
Please answer both of the questions. Please write neatly and
also make sure you answer is correct! Thank you! If you do not know
the answer to a question, please don’t answer it! I will rate
good!
12. (2 pts) Which one of the following statements is FALSE? A. In feedback inhibition, the product of a pathway inhibits an early enzymatic step. B. Protein kinases add inorganic phosphate (P) to a Ser, Thr, or Tyr C. An enzyme could be...
5. When all of the sample to be collected has left the column, will the drop...
5. When all of the sample to be collected has left the
column, will the drop of solution coming off the tip of the column
be acidic, basic or neutral?
6. How many H+ ions would be displaced from the ion
exchange column by 1 Cu2+ ion?
7. Binding sites on the ion exchange resin are saturated
with which ion before the sample solution is added?
8. Ions are displaced from the resin binding sites by
any ion that has...
4. (Challenge) You are purifying a protein from the brain of patients with an unusual type of...
4. (Challenge) You are purifying a protein from the brain of patients with an unusual type of dementia triggered by a coronavirus and you hypothesize that this protein may be a signature molecule of this type of dementia. To pursue this hypothesis, you collect brain tissue from patients post mortem, do differential centrifugation, density gradient centrifugation, CM (carboxymethyl cellulose) ion exchange chromatography and gel filtration. You then assess the purity of your protein using both native and SDS gel electrophoresis. The...
acetyl-CoA COA The reactions of the citric acid cycle are shown in the image. As labeled in the diagram, reactions 1, 3, and 4 are regulation points in the citric acid cycle. oxaloacetate citrate synthase citrate NADH +Hi 7 malate dehydrogenase NAD malate aconitase 2 Which molecule inhibits reaction 1? isocitrate 7 fumarase NAD isocitrate dehydrogenase 3 NADH + H+ O citrate O NAD acetyl-CoA + CO2 fumarate C-ketoglutarate FADH succinate O dehydrogenase FAD O-ketoglutarate a dehydrogenase NAD + COA...
Part B Use the structures given in the cycle shown before this part identify the enzymes involved in each step of the citric acid cycle by completing each sentence Match the words in the left column to the appropriate blanks in the sentences on the right. View Available Hint(s) Reset Help isocitrate dehydrogenase aconitase citrate synthase fumarate hydratase succinate dehydrogenase succinyl-CoA synthetase a-ketoglutarate dehydrogenase malate dehydrogenase In step 1, oxaloacetate condenses with acetyl-CoA to form citrate, which is catalyzed by...
12. Which enzyme catalyzes the reaction shown? Oo 9% a. succinyl-CoA synthetase b. succinate dehydrogenase c. pyruvate dehydrogenase d、a-ketoglutarate dehydrogenase 13. Which of the reactions of the citric acid cycle requires FAD as a coenzyme? a. the conversion of isocitrate to a-ketoglutarate b. the conversion of citrate to isocitrate c. the conversion of succinate to fumarate d. the conversion of malate to oxaloacetate 14. Another name for Complex I in the electron transport chain is a. cytochrome c oxidase. b....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
just checking my answers, except for number 10.
The common molecule produced from all foods at the second stage of catabolism is A) ADP. B acetyl-CoA. C) glucose. D) citric acid. Which group of compounds includes only low-energy molecules? 1. 2. Hih A)ATP; acetyl-CoA; NADH; FADH2 C) ADP: CO2; NADH; FADH2 DONO BADP: acetyl-CoA; NAD+:FAD-mau ADP: CO2: NAD+, FAD After O2 has been reduced in the electron transport chain, the oxygen atoms are part of what molecules? A) ATP 3....
Question 3. Draw the tripeptide: Val-Ser-Ala. Include a phosphoryl group on the amino acid sidechain that is most likely to undergo phosphorylation. Question 4. A protein retained on an ion exchange chromatography column is usually eluted off the column by (circle the correct answer) A. increasing the salt concentration in the buffer B. adding the protein's free ligand. C. changing the temperature of the elution buffer. D. allowing the retained protein to naturally come off the column after the non-specifically...
Please answer both of the questions. Please write neatly and
also make sure you answer is correct! Thank you! If you do not know
the answer to a question, please don’t answer it! I will rate
good!
12. (2 pts) Which one of the following statements is FALSE? A. In feedback inhibition, the product of a pathway inhibits an early enzymatic step. B. Protein kinases add inorganic phosphate (P) to a Ser, Thr, or Tyr C. An enzyme could be...
5. When all of the sample to be collected has left the
column, will the drop of solution coming off the tip of the column
be acidic, basic or neutral?
6. How many H+ ions would be displaced from the ion
exchange column by 1 Cu2+ ion?
7. Binding sites on the ion exchange resin are saturated
with which ion before the sample solution is added?
8. Ions are displaced from the resin binding sites by
any ion that has...
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