What do you think should happen to DNA at 1 K (temp)?
DNA become very stable at such low temperature like 1 Kelvin and this kind of technique is used mainly for preservation and storage of DNA for long period of time. For example, DNA required for testing a pharmaceutical product will need to be stable for a few years, whereas samples used in evolutionary biology have been in existence for millions of years.
At such low temperature DNA is maintained in a glassy (or vitreous) state. In the glassy state, molecules lose the ability to diffuse such that the movement of a proton is estimated to be approximately one atomic diameter in 200 years, thereby preventing chemical and nuclease degradation.
Basically at such low temperature, conformation of folding of DNA is very stable, dense and unreactive.
EXPERIMENT 1: What do you think will happen to the temperature if you used 75 mL of HCl and 75 mL of NaOH instead of the 10 mL each you used in Experiment 1?
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