Describe the main features of an eukaryotic RNA Polymerase II complex with all 12 subunits identified from the crystal structure, and the function of each feature. ) Describe the main features of an eukaryotic RNA Polymerase II complex with all 12 subunits identified from the crystal structure, and the function of each feature.
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Describe the main features of an eukaryotic RNA Polymerase II complex with all 12 subunits identified...
Suppose a mutation occurs in the gene encoding eukaryotic RNA polymerase I, II, or lll that renders that polymerase non-functional. Match each RNA polymerase mutation with all of the cellular processes that it would disrupt. Mutation in eukaryotic RNA polymerase I Mutation in eukaryotic RNA polymerase II Mutation in eukaryotic RNA polymerase III pre-mRNA processing RNA synthesispre-mRNA synthesis RNAi-mediated gene regulation IRNA synthesis mRNA translation rRNA processing
b) Draw an elongating RNA polymerase. Label all features, enzymes, and molecules we discussed in class. Describe the different functions RNA polymerase is performing (10 words per function) (25 points) b) Draw an elongating RNA polymerase. Label all features, enzymes, and molecules we discussed in class Describe the different functions RNA polymerase is performing (10 words per function) (25 points) b) Draw an elongating RNA polymerase. Label all features, enzymes, and molecules we discussed in class Describe the different...
Can someone please help me answer these questions. Thank you! Eukaryotic transcription signals a) This drawing shows the placements of the four main sequences of the eukaryotic core promoter for RNA polymerase II. Identify each one and give a brief explanation b) Which sequences are used in a DPE-driven promoter? c) Which ones are used in a TATA-driven promoter? d) Please draw and describe the steps as the transcription factors work with eukaryotic RNA polymerase II to start transcription of...
Describe in detail the structure of the RNA polymerase II CDT and how the structure is used to sequentially regulate the maturation of the pre-mRNA during the elongation phase of transcription. Focus on the events which happen on RNA polymerase II CDT. For mechanisms which are directly responsible for pre-mRNA processing, you only need to mention their names.
What are the four common core promoter elements for eukaryotic genes transcribed by RNA polymerase II? Must all of these elements be present in the promoter for transcription of every gene to occur? Explain
Why does E. coli have several different sigma factors? A.They allow RNA polymerase I, RNA polymerase II and RNA polymerase III to bind to different promoters. B.They allow the different subunits of the RNA polymerase holoenzyme to bind to each other. C.They are redundant in case one is mutated. They all perform the same function. D.One is needed to transcribe mRNA. A second is needed to transcribe tRNA. And a third is needed to transcribe rRNA. E.They are used if...
on and 6. Draw the same Eukaryotic transcript after splicing has removed the m label the remaining parts. 7. Describe the roles of the following features involved in Eukaryotic transcription: TATA Binding Protein General Transcription Factors Polymerase II 8. In Eukaryotic transcription, which happens first? A. The General Transcription Factors are recruited, and the Preinitiation complex (PIC) is assembled. B. The TBP binds the promoter 9. Describe the carboxy terminal domain (CTD) of RNA polymerase II and two of its...
Which of the following is NOT a function of transcription that requires the activity from subunits of the Core RNA Palymerase? a. RNA polymerase activity that base-pairs and polymerizes nucleotides to make mRNA. b. Helicase activity that unwinds the double-stranded DNA molecule for transcription c. Specific recognition of -35 box and -10 box sites in the promoter region. d. General binding that helps RNA polymerase loosely adhere to DNA, before Transcription begins. Oe. Trick Question. The Core RNA polymerase can...
In a eukaryotic cell, the hydrophilic ligand CUE binds the enzyme-linked receptor CUE receptor. Describe a hypothetical but realistic signal transduction cascade, that eventually leads to the transcription of the GRAD gene. The GRAD protein is secreted by the cell. Describe the critical steps in gene transcription, RNA processing and in translation, and which structures and organelles are involved. A detailed description of transcription and translation, and all proteins involved, is NOT expected, but a description of the main steps...
Identify the statements that are features of a promoter. Pick all that Apply. A. In eukaryotes, the promoter recruits the preinitiation complex, which includes the TATA-binding protein. b. In prokaryotes, the promoter is recognized by general transcription factors (GTF), which recruit the RNA polymerase holoenzyme. c. In eukaryotes, the promoter attracts the small and large ribosomal subunits with the help of initiation factors. d. In prokaryotes, the promoter contains a –35 and –10 region upstream of the transcription start site...