In order to understand the function of a gene during a CRISPR experiment, why is it...
In order to understand the function of a gene during a CRISPR experiment, why is it preferable to introduce a mutation early in its sequence?
Homework Answers
The alteration in a gene sequence alters it's expression. Therefore in order to understand the function of a gene during a CRISPR experiment, it is preferable to introduce a mutation early in its sequence so that the changes are observed when the gene is expressed. This method is very useful to rectify gene defects by modifying it through introduction of a useful mutation.
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In order to understand the function of a gene during a
CRISPR experiment, why is it...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do bacteria use CRISPR? 4.How is the enzyme targeted to a specific DNA sequence? 5.what need to be done in order to make sure the proper human protein is made in bacteria cells? 6.How can you check for proper orientation of the inserted DNA in the plasmid? 7. Why are iPSCs useful for studying hereditary neuronal diseases such as Alzheimer's. 8. How are pig cells...
Question 1 Question 2: Loss-of-function mutations in the CCR5 gene have been linked to resistance to...
Question 1
Question 2:
Loss-of-function mutations in the CCR5 gene have been linked to resistance to HIV infection. As a researcher you are interested in using CRISPR to provide protection against HIV. Provide a brief overview of the steps you would take to use CRISPR target the CCR5 gene in order to give someone resistance to HIV. Be sure to identify the main biochemical components, their function, and the cellular processes directly involved. A mutation in the RB gene prevents...
part of it is I also have to explain why all the options that are incorrect...
part of it is I also have to explain why all the options that are
incorrect are incorrect
W A Strands, and it has a fued mutation rate. Question 4 1 pts In a strain of bacteria, a mutation changes the sequence of repeat 2 in the CRISPR gene (see figurel. How will this affect bacterial immunity? • This is an analysis question because you have to break the CRISPR-Cas system into its component pleces to understand how a mutation...
Suppose you used CRISPR/Cas9 to target a gene without providing a repair template, and that this...
Suppose you used CRISPR/Cas9 to target a gene without providing a repair template, and that this experiment results in a deletion of one DNA base pair right after the ATG, of the gene you are studying. How might this affect protein function?
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
please help answer these questions 3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
understand beadle and tatums experiment using neurospora arginine auxotrophs. why their hypothesis "one gene one enzyme"...
understand beadle and tatums experiment using neurospora arginine auxotrophs. why their hypothesis "one gene one enzyme" is now dicarded
Question 1: What was determined to be the function of the CRISPR loci in microbes? Question...
Question 1: What was determined to be the function of the CRISPR loci in microbes? Question 2: Explain the differences in the roles of the cas7 and cas9 gene. Question 3: What genetic material does CRISPR target? Question 4: Due to the ability of CRISPR to cleave DNA sequences at specific sites, it is considered a programmable version of what? Question 5: Define and explain the significance of the PAM sequence. Question 6: What is the role of tracrRNA in...
Two CRISPR experiments (A and B) have been conducted in yeast cells to understand the effect...
Two CRISPR experiments (A and B) have been conducted in
yeast cells to understand the effect of inducible promoters. The
yeast cells used in both experiments contain 2 plasmids: one
plasmid that has the Cas9 gene and one plasmid that has the CAN1.Y
gRNA sequence. However, the two experiments differ in the promoter
used in one of the plasmid.
The graph below shows the colony count from the
canavanine selection plates (YNB + met + his + canavanine) that use...
why people can use T7 Endonuclease I to detect the editing of targeted gene by CRISPR/Cas9?...
why people can use T7 Endonuclease I to detect the editing of targeted gene by CRISPR/Cas9? Please explain the principle.
Question 1
Question 2:
Loss-of-function mutations in the CCR5 gene have been linked to resistance to HIV infection. As a researcher you are interested in using CRISPR to provide protection against HIV. Provide a brief overview of the steps you would take to use CRISPR target the CCR5 gene in order to give someone resistance to HIV. Be sure to identify the main biochemical components, their function, and the cellular processes directly involved. A mutation in the RB gene prevents...
part of it is I also have to explain why all the options that are
incorrect are incorrect
W A Strands, and it has a fued mutation rate. Question 4 1 pts In a strain of bacteria, a mutation changes the sequence of repeat 2 in the CRISPR gene (see figurel. How will this affect bacterial immunity? • This is an analysis question because you have to break the CRISPR-Cas system into its component pleces to understand how a mutation...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
Two CRISPR experiments (A and B) have been conducted in
yeast cells to understand the effect of inducible promoters. The
yeast cells used in both experiments contain 2 plasmids: one
plasmid that has the Cas9 gene and one plasmid that has the CAN1.Y
gRNA sequence. However, the two experiments differ in the promoter
used in one of the plasmid.
The graph below shows the colony count from the
canavanine selection plates (YNB + met + his + canavanine) that use...
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