how is transforming trna in a pcr reaction helpful?
Homework Answers
Transforming tRNA in a PCR reaction is helpful. The primers used in PCR can amplify tRNA genes for cloning which can further be sequenced. This can be done by using conserved regions of genes coding for the structural portion of tRNA. Thus, sequence variations of individual genes and identification of new families containing tRNA genes can be detected by the PCR.
Describe how the polymerase chain reaction (PCR) works. What are the three steps in a PCR...
Describe how the polymerase chain reaction (PCR) works. What are the three steps in a PCR cycle and what is happening at each step?
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
Polymerase chain reaction (PCR): The gold standard of nucleic acid amplification The polymerase chain reaction (PCR)...
Polymerase chain reaction (PCR): The gold standard of nucleic acid amplification The polymerase chain reaction (PCR) is a powerful tool for amplifying genetic material (that is, making many copies of it). This technique can be used for a wide range of applications, from examining and manipulating the specific genes involved in making L. monocytogenes pathogenic to analyzing the phylogenetic relationships between microbes. What are the steps of PCR required to amplify the recombinant DNA? Drag and drop the events into...
1. What is the purpose of the PCR nucleotide mix added to your PCR reaction? 2....
1. What is the purpose of the PCR nucleotide mix added to your PCR reaction? 2. What is the purpose of Gel Green? What type of chemical is it? Molecularly how does it work? 3. Why are the restriction digest reactions mixed by flicking or pipetting, rather than vortexing? 4. In the ligation, hAPP can only insert in one orientation into the vector why is this? What is the role of hAPP in DNA replication? What is its role in...
a. List the six main components of a PCR amplification reaction and explain how its preseneel...
a. List the six main components of a PCR amplification reaction and explain how its preseneel master mix is need for the production of an amplicon. Components Template DNA Primers MgCl2 dNTP Buffer Polymerase Why it is needed on
qRT-PCR The reaction mixture for qRT-PCR requires the following: Taq Mix : 1 uL, SYBR Green...
qRT-PCR The reaction mixture for qRT-PCR requires the following: Taq Mix : 1 uL, SYBR Green Mix: 25 uL, Forward primer : 1 uL, Reverse primer : 1 uL, ROX Reference Dye : 1 uL, Template : 50 ng RNA , Volume up to 50 uL with nuclease free water. If you are going to make a 20 uL reaction mixture and have a 10 ng/uL RNA sample, how much of each of the components do you need? You also...
Suppose you start a PCR reaction with 3 copies of a double stranded DNA fragment. How...
Suppose you start a PCR reaction with 3 copies of a double stranded DNA fragment. How many copies will be present after 4 replication cycles? a. 7 b. 64 c. 48 d. 24 e. 8
7. You are making a PCR reaction mixture with a total volume of 50uL.( THIS IS...
7. You are making a PCR reaction mixture with a total volume of 50uL.( THIS IS IN MICROLITERS NOT MILILITERS AS ANOTHER QUESTION ON CHEGG HAS ALREADY BEEN POSTED BUT IT STATES THE TOTAL VOLUME AS 50 mL.) The primer set concentration is 1μl primer/10μl reaction volume. The plasmid stock solution concentration = 0.01μg/μl, of which you need 100ng. How much of each PCR reaction component will you add, given the following? 5x Mastermix __________ Primer set __________ DNA template...
Is it possible for bacterial cell components used in translation such as Ribsomes, tRNA, and aminos...
Is it possible for bacterial cell components used in translation such as Ribsomes, tRNA, and aminos acids, to be used with the addition of eukaryotic mRNA to produce a protein in the PCR system? Please explain as much as you can!
7. List the components (ingredients) required for a PCR reaction. 8. List the three main steps...
7. List the components (ingredients) required for a PCR reaction. 8. List the three main steps of a PCR reaction in order, from start to finish, of one eyele. 9. In addition to the components required for PCR, what is additionally required for Sanger sequencing, and why (what does it do)? | 10. Approximately how 'big' was the piece of DNA that we amplified? 11. Our DNA samples were quantified using a fluorometer. When we received the quantification results, what...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
Polymerase chain reaction (PCR): The gold standard of nucleic acid amplification The polymerase chain reaction (PCR) is a powerful tool for amplifying genetic material (that is, making many copies of it). This technique can be used for a wide range of applications, from examining and manipulating the specific genes involved in making L. monocytogenes pathogenic to analyzing the phylogenetic relationships between microbes. What are the steps of PCR required to amplify the recombinant DNA? Drag and drop the events into...
a. List the six main components of a PCR amplification reaction and explain how its preseneel master mix is need for the production of an amplicon. Components Template DNA Primers MgCl2 dNTP Buffer Polymerase Why it is needed on
7. List the components (ingredients) required for a PCR reaction. 8. List the three main steps of a PCR reaction in order, from start to finish, of one eyele. 9. In addition to the components required for PCR, what is additionally required for Sanger sequencing, and why (what does it do)? | 10. Approximately how 'big' was the piece of DNA that we amplified? 11. Our DNA samples were quantified using a fluorometer. When we received the quantification results, what...
Most questions answered within 3 hours.
-
Kylie is a single mom with two dependent children,
Tanner, age 7 and Olivia, age 11....
asked 22 minutes ago -
Phosphorous + bromine = phosphorous tribromide. If 35.0 g of
bromine are reacted and 27.9 grams...
asked 1 hour ago -
Derive the long wavelength limit of the Planck energy density
distribution
asked 1 hour ago -
Calculate the pH of each of the following solutions.
0.50 M HBr
3.1×10−4 M KOH
4.2×10−5...
asked 5 hours ago -
For the year ended December 31, Depot Max’s cost of merchandise
sold was $85,600. Inventory at the...
asked 5 hours ago -
Week 10 - Professional Memo Assignment
Professional Memo Assignment
Your mission for this week, should you...
asked 5 hours ago -
Write a Python program that stores the data for each
player on the team, and it...
asked 5 hours ago -
In
the last 3 months, mike never knows when he is going to get his
allowance...
asked 5 hours ago -
Is Ca(OH)2 a Bronsted base, Lewis base, or both? Why?
asked 5 hours ago -
1A- Why don’t voters complain about U.S. tariffs on imported
sugar?
Because sugar is only a...
asked 5 hours ago -
Cash Payback Period
Primera Banco is evaluating two capital investment proposals for
a drive-up ATM kiosk,...
asked 5 hours ago -
Create a button in Swift (Xcode) that will create a charge,
create a charge using Stripe's...
asked 5 hours ago