Question 1: Describe 2 approaches that you can use to determine that you have successfully PCR...
Question 1: Describe 2 approaches that you can use to determine that you have successfully PCR amplified Sonic Hedgehog.
Homework Answers
1. Gel electrophoresis. - After PCR amplification Gel electrophoresis is the easiest way to confirm the PCR products through molecular weight. Run the PCR products in the gel shows the based on molecular weight of Sonic Hedgehog gene it will stop at the same range and confirm it successfully amplified the Sonic Hedgehog gene.
2. DNA probe hybridization - It is more sequence specificity and tells the amplified product is the Sonic Hedgehog gene or not.
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Question 1: Describe 2 approaches that you can use to determine
that you have successfully PCR...
D. After you run your PCR reaction, what next steps would you perform to determine that...
D. After you run your PCR reaction, what next steps would you perform to determine that the PCR amplified the region of interest? E. After your procedures in step D above, you decide to measure the concentration of DNA in your PCR product. Briefly explain how you would do this given the tools we have available. What values would you expect to confirm that you have a good DNA concentration and purity? F. Write and briefly describe a program you...
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help please
Question 31 (1 point) Saved For the most fundamental form of PCR, what do you need: 1) DNA of interest 2) 15-30 base pairs of known sequence flanking the DNA to be amplified 3) A vector 4) Some way to change the temperature of the reaction 1, 3, and 4 O 1,2,3, and 4 1,2, and 4 1,2, and 3
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D. After you run your PCR reaction, what next steps would you perform to determine that the PCR amplified the region of interest? E. After your procedures in step D above, you decide to measure the concentration of DNA in your PCR product. Briefly explain how you would do this given the tools we have available. What values would you expect to confirm that you have a good DNA concentration and purity? F. Write and briefly describe a program you...
rate for answering questions
compeletly.
You plan to use a PCR based assay to determine whether 3 mosquitoes that you have collected are infected with West Nile virus. You have available to you three different virus-specific primers. The primers recognize sequences at the following nucleotide positions on the viral genome: primer 1: 155-176 primer 2: 745-720 primer 3: 926-905 The 5' and 3' ends of the genome are indicated on the long line The first nucleotide at the 5' end...
Lab 1 - PCR Pre-lab discussion. Discuss the following among your table prior to beginning the lab. 1. Describe the overall goal of the polymerase chain reaction. 2. List the components that are required for a PCR reaction. 3. A special DNA polymerase called Taq is used in PCR. What is unique about this enzyme and why is it used in the PCR reaction? 4. We will be using genomic DNA as our template DNA in this PCR reaction. How...
help please
Question 31 (1 point) Saved For the most fundamental form of PCR, what do you need: 1) DNA of interest 2) 15-30 base pairs of known sequence flanking the DNA to be amplified 3) A vector 4) Some way to change the temperature of the reaction 1, 3, and 4 O 1,2,3, and 4 1,2, and 4 1,2, and 3
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