Question

Match the biotechnology method with its description

separates molecules based on size and charge

starts with lysis of cells

makes copies of specific DNA regions

detects the presence of specific DNA sequences

             detects the expression of specific genes

Descriptions: DNA Extraction

                          polymearase chain reaction

                          Southern blotting

                          gel electrophoresis

                          Northern blotting

Answer 1

separates molecules based on size and charge= gel electrophoresis ( separates molecules based on size and charge in presence of electric field)

starts with lysis of cells= DNA Extraction ( cell lysis is necessary to open up DNA from cell nucleus)

makes copies of specific DNA regions = polymearase chain reaction (PCR is in vitro form of DNA replication)

detects the presence of specific DNA sequences= Southern blotting (radio labeled probes are used to detect specific DNA sequence, probes are synthetic known sequence of DNA which is complementary to target DNA sequence)

             detects the expression of specific genes = Northern blotting ( it is used to detect presence of RNA, when gene express then it produce mRNA and this mRNA are detect in this method)