In what order will the following proteins elute from a Sephadex G-150 column: catalase (222 kDa),...
In what order will the following proteins elute from a Sephadex G-150 column: catalase (222 kDa), chymotrypsin (21.6 kDa), concanavalin B (42.5 kDa), lipase (6.7 kDa), and lysyl oxidase (30 kDa)?
Homework Answers
In gel filtration chromatography, separation of molecules is achieved on the basis of their size in such a way that small molecules enter many pores in the gel media, equilibrating between the gel and moving buffer and so travel slowly and hence eluted later whereas large molecules enter few pores and thus travel faster and are eluted earlier.
Hence in the given question, gel using Sephadex G-150 whose range is 5-300 kDa,
The order of elution will be in the decreasing order of molecular size as
1st-Catalase-222 kDa
2nd-Concanavalin-42.5 kDa
3rd-lysyl oxidase-30 kDa
4th-Chymotrypsin-21.6 kDa
5th-lipase-6.7 kDa
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In what order will the following proteins elute from a Sephadex
G-150 column: catalase (222 kDa),...
In what order would the proteins elute from a size exclusion column (limit 50- 40,000)? Protein...
In what order would the proteins elute from a size exclusion column (limit 50- 40,000)? Protein Molecular weight 1- 90 KDa 2- 27,000 KDa 3- 300 KDa
Which of these 5 proteins will elute first from the gel filtration (size exclusion) column? Serum albumin (68 kDa) a-la...
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9. In the mixture of proteins you have protein A: 35 kDa, protein B: 15 kDa,...
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#2 is Q HiTrap column where it is anion exchange column not cation 2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrin...
#2 is Q HiTrap column where it is anion exchange column not
cation
2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
The five proteins listed below were run on a cation exchange column at pH = 7...
The five proteins listed below were run on a cation exchange column at pH = 7 Cytochrome C pl = 10.7 Ribonuclease A pl = 9.5 Myoglobin pl = 7.4 Carbonic anhydrase pl = 6.0 Glucose oxidase pl = 4.2 Which of the following statements is TRUE? A) Glucose oxidase and carbonic anhydrase will be separated in the low salt wash b) Cytochrome C binds and then elutes first when the salt concentration is increased c) decreasing the pH will...
e stwc snct 2pts. Caci 1. In order to elute Con A from the affinity beads,...
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NOTES: To separate on a size-exclusion column, differences in MW need to be greater than 10%....
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9. The following proteins were identified in a mixture. protein te precipitate MW (kDa) 4.8 22...
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what is the principle of gel filtration chromatography? 19 E 3 2 1 AaBbceDdEe ABCDdee AaBbCcDc...
what is the principle of gel filtration chromatography?
19 E 3 2 1 AaBbceDdEe ABCDdee AaBbCcDc AaBbCcDdE No Spacing Heading 1 Heading 2 7. A. What is the principle of Gel filtration chromatography? (1 point) B. In gel filtration chromatography, what is the stationary phase and the mobile phase (that we used in our lab experiment)? (1 point) C. In what order would the proteins elute from a size exclusion column (limit 50- 40,000) (1 point) Protein Molecular weight KDa...
a. Which spot corresponds to the protein with the largest molecular weight? b. When pH = 7, what order would the follow...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
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Which of these 5 proteins will elute first from the gel filtration (size exclusion) column? Serum albumin (68 kDa) a-lactalbumin (14.2 kDa) O Lactoferrin (87 kDa) k-casein (19 kDa) O b-lactoglobulin (18 kDa) O Glucagon activation of a G protein-coupled membrane receptor (GPCR) induces GTP binding to which of the following membrane anchored proleins? Gbg Phospdlipase A2 None of the mentioned options The GPCR itself Ga Which of the following sphingolipids is a phospholipid? Sphingomyelin Gangliosides None of the mentioned...
9. In the mixture of proteins you have protein A: 35 kDa, protein B: 15 kDa, protein C: 58 kDa, protein D: 150 kDa. Propose the chromatographical method to separate them. Write in which order they would elute from the column. 10. What is the best chromatographical method to separate fluorescein and amide black? They are organic dyes.
#2 is Q HiTrap column where it is anion exchange column not
cation
2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
e stwc snct 2pts. Caci 1. In order to elute Con A from the affinity beads, (a) 1.0M NaCI was used, (b) HRP was used, (c) the sephadex beads were washed twice with NaCl, (d) glucose was used, (e) the elution buffer contained a carbohydrate binding protein. 2. A specific biological property is used to separates macromolecules in (a) affinity chromatography (b) TLC, (c) ion exchange chromatography, (d) electrophoresis, (e) protein assay 3. In order to separate proteins from lectins...
9. The following proteins were identified in a mixture. protein te precipitate MW (kDa) 4.8 22 IMRSO) 45% 80% 65% 20% 30% 45% 5.3 6.8 9.50 un 115 5.3 th(cont.) a) What is meant by "salting out" a protein using MgSO.? What happens on a molecular level? b) Draw a gel indicating proteins 1-6 order of elution in SDS-PAGE. Label where sample would start so I am clear on the validity of your answer. c) Draw their order of elution...
what is the principle of gel filtration chromatography?
19 E 3 2 1 AaBbceDdEe ABCDdee AaBbCcDc AaBbCcDdE No Spacing Heading 1 Heading 2 7. A. What is the principle of Gel filtration chromatography? (1 point) B. In gel filtration chromatography, what is the stationary phase and the mobile phase (that we used in our lab experiment)? (1 point) C. In what order would the proteins elute from a size exclusion column (limit 50- 40,000) (1 point) Protein Molecular weight KDa...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
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