Question

As we saw in prior chapters, metals can play a variety of roles in catalysis. What are the roles of magnesium ion in hexokinase, glucokinase, and phosphoglycerate kinase?

		The Mg helps hold the enzyme in the correct conformation.
		The Mg helps hold ADP/ATP in the correct conformation.
		The Mg helps hold the substrate in the correct connformation
		It serves as a REDOX partner.
		It helps stabilize the ES complex by balancing charge in the active site
		It coordinates to the substrate carbonyl making it a better electrophile

More than one answer may be correct

Answer 1

Right ans is- It helps stabilize the ES complex by balancing charge in the active site

because The bivalent metal assists the enzyme ligands in shielding the bound phosphate group's negative charges, allowing the nucleophilic attack to occur; this charge-stabilization is a typical characteristic of phosphotransfer reaction.