Question

What is the purpose of SDS? ***To Make the protein negative was not an option

What will happen if you remove SDS from SDS PAGE?

What are the products of light dependent and light independent reactions?

Why does absorbance decrease when you add DCPIP to cholorplast and use light ?

Where would you find LHCII ?

Which of the following is true about abtibodies? None!

What will happen if you put the gel against the positive pole and the membrane against ghe negative pole?

What is the purpose of blocking?

What is the order of immunoblotting?

Why is immunoblotting better than using Commassie Blue?

If an herbecide was used and only co2 was generated and ATP was not generated, what was the mode of action of herbecide?

Answer 1

1) Purpose of SDS is to allow the separation of proteins on the basis of mass only. It renders the intrinsic charge of proteins insignificant by giving them all net negative charge. SDS binds to protein in proportion to the protein's molecular weight and alters its native confirmation. So, proteins carry same charge, assume same shape thereby, allowing their separation on the basis of molecular weight only.

2) If SDS is removed from SDS PAGE, different native charge and confirmation of different protein would not allow their separation on the basis of molecular weight only.

3) Products of light dependent reactions: ATP, NADPH, oxygen (By-product)

Products of light independent reactions: Glucose and water.