A PCR reaction uses 2 primers (a forward primer and a reverse primer). Describe what happens...
A PCR reaction uses 2 primers (a forward primer and a reverse primer). Describe what happens to the concentration of these primer populations during a successful PCR reaction (hint: do they increase, decrease, or stay the same? Explain.)
Homework Answers
There are 2 set of primers used in polymerase chain reaction as a forward and other is reversed. The concentration of these primer pairs during the successful PCR reactions are decreases because primers are annealing to the gene target and further by the help of dNTPs and DNA polymerase it involves in the amplification of DNA fragment. So, of primer Will bind with DNA, it will be utilized in PCR reactions and the concentration will be decreases.
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A PCR reaction uses 2 primers (a forward primer and a reverse
primer). Describe what happens...
design a forward and reverse PCR primer based on underlined sequence in the following DNA sequence....
design a forward and reverse PCR primer based on underlined sequence in the following DNA sequence. 3' AAGCCAGGCCCTGGAGT......................TGGACTCGTGGGTGTG.....5' What does "PCR stand for and briefly describe PCR program
Prepare a PCR reaction using the following recipe. 5x buffer..............................5 μl 25 μM forward primer..........2 μl,...
Prepare a PCR reaction using the following recipe. 5x buffer..............................5 μl 25 μM forward primer..........2 μl, 25 μM reverse primer..........2 μl 5 ng/μl Template..................1 μl 2.5 mM (each) dNTPs..........4 μl Water....................................30 μl Phusion polymerase.............1 μl Q6. What are the final concentrations of the buffer, the forward primer, the template, and the dNTPs in the PCR reaction? Leave all concentrations in the same type of units they were provided in (x, molarity, or weight per volume). Remember the equation C1 x...
To do the PCR, we used 2 universal primers that roughly match sequences near the 5’...
To do the PCR, we used 2 universal primers that roughly match sequences near the 5’ and 3’ ends of the 16S rRNA gene. The forward primer binds near the 5’ end to the complementary strand of the sequences shown below. The sequence of the forward primer is: Agagtttgatcctggctcag The reverse primer binds near the 3’ end to the sequences shown below. The sequence of the reverse primer is: ACGGCTACCTTGTTACGACTTG To find the primer in the sequence below, you must...
Forward primer 5'-ACCACCCAGCCCTTAGTGACCAGCTA-3' Reverse primer 5'-AAAGTTTTATTCATTGCAGAGGGGT-3' GAATTCTGTAATAACTATATAGAACTCTTCTTATATATGCTCAAATTTTA CATGCTAGCCTTCAGGTACATATCTTGGGTTGTTGGGTATTGTAGAAGAA TGTACTACAGGGCTTCAGCCCAGTTGACCAATGAGTGGGCTACGGGGTTT GTGAAAG
Forward primer 5'-ACCACCCAGCCCTTAGTGACCAGCTA-3' Reverse primer 5'-AAAGTTTTATTCATTGCAGAGGGGT-3' GAATTCTGTAATAACTATATAGAACTCTTCTTATATATGCTCAAATTTTA CATGCTAGCCTTCAGGTACATATCTTGGGTTGTTGGGTATTGTAGAAGAA TGTACTACAGGGCTTCAGCCCAGTTGACCAATGAGTGGGCTACGGGGTTT GTGAAAGGAGAGATGGAGAAGGAGGGACCATTAAGTACCTTGCTGCCTGA GTTCTGCTTTCCTCCTCCCTCTGAGGGTGAGCTGGGATCTCATCTGAGTT AAGGGCCCAGCTATCAATGGGAACTGTGAAACAGTCCAAGGGACATCAAT ATTAGGTCCCTAACAACTOCAGTTTCCTGGGGAATGATGTGGAAAATGCT CAGCCAAAGATGAAGAAGGTCTCACCTTCTGGGACAATGTCCCCTGCTOG GAACTGGTTCATCAGGCCATCTGGTCCCTTATTAAGACTATAATAACCCT AAGACTAAGTAGATGTGTTGATGTCCAATGAGTGCTTTCTGCAGACCTAG CACCAGGCAAGTGTTTGGAAACTGCAGCTTCAGCCCCTCTGGCCATCTGC CTACCCACCCCACCTGGAGACCTTAATGGGCCAAACAGCAAAGTCCAGGG GGCAGAGAGGAGGTACTTTOGACTATAAAGCTGGTGGGCATCCAGTAACC CCCAGCCCTTAGTGACCAGCTATAATCAGAGACCATCAGCAAGCAGGTAT GTACTCTCCTCTTTGGGCCTGGCTCCCCAGCCAAGACTCCAGOGACTTTA GGGAGAATGTGGGCTCCTCTCTTACATGGATCTTTTGCTAGCCTCAACCC TGCCTATCTTTCAGGTCATTGTTTCAACATGGCCCTGTTGGTGCACTTOC TACCCCTGCTGGCCCTGCTTGCCCTCTGGGAGCCCAAACCCACCCAGGCT TTTGTCAAACAGCATCTTTGTGGTCCCCACCTGGTAGAGGCTCTCTACCT GGTGTGTGGGGAGOGTGGCTTCTTCTACACACCCAAGTOCCGCOGTGAAG TGGAGGACCCACAAGTGGAACAACTGGAGCTGGGAGGAAGCCCOGGGGAC CTTCAGACCTTGGCGTTGGAGGTGGCCOGGCAGAAGCGTGGCATTGTGGA TCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCA ACTAAGGCCCACCTCGACCOGCCCCACCCCTCTGCAATGAATAAAACTIT TGAATAAGCACCAAAAAAAAGAGTTCTATAATGAATGAAAAAGGATIGTG TATATAGACATCTTTTTCTCTGGCATTTATTGTCATGTTAGCATACTATT AAACCATTGTTAGGTTGGATGATTATATAATCATGTATGAAGCTTGTGAT AAAACACCAGGAATAATTCAAGTATCTGGAATTC 3 Question: 1) Predict the sizes of the PCR products. 2) Draw the DNA primers base pairing at genomic sequence.
What is the purpose in a PCR reaction for each of the following reagents? Taq Taq...
What is the purpose in a PCR reaction for each of the following reagents? Taq Taq buffer dNTPs Forward primer Reverse primer Genomic DNA What do you think would happen if you forgot to add your Reverse primer when you did a PCR?
Primers are short pieces of DNA that are used in PCR (Polymerase Chain Reaction), the process...
Primers are short pieces of DNA that are used in PCR (Polymerase Chain Reaction), the process which is used in molecular labs to make copies of short stretches of a gene or genome. You have designed primes that have a Molecular Weight (MW) of 6000. It arrives as a dry sample in the quantity of 300 µg. How much water do you need to add to make a primer stock solution of 100 mM primers? HINTS: Remember that mM is...
1. What are the degenerate primers in the PCR amplification protocol? What do they do? 2....
1. What are the degenerate primers in the PCR amplification protocol? What do they do? 2. Suppose you begin a PCR reaction with 1 piece of double stranded DNA. After 30 cycles of replication, how many pieces of double stranded DNA do you now have? 3. What would be the consequence of having too much DNA in the sample? Would it interfere with the PCR reaction? Why? 4. What happens during the annealing step of the PCR reaction? During the...
1.) How many kinds of reverse transcriptases are there ? 2.) What would normally be a...
1.) How many kinds of reverse transcriptases are there ? 2.) What would normally be a proper order to assemble yout RT reaction mix ? Your PCR mix ? Why is the order important ? 3.) Why do you need many PCR cycles to see reaction products ? 4.) Why are multiple product bands sometimes seen during PCR reactions ? How can this problem be minimized ? 5.) Betaine is often included in transcription reactions. What is betaine ? What...
please help me!!!!!!!!! do both i need help Dz. You are creating PCR primers for the...
please help me!!!!!!!!!
do both i need help
Dz. You are creating PCR primers for the DNA sequence pictured. If the given sequence is the plus strand, what would be the reverse primer? (Type the primer in 5' to 3' orientation without spaces) 5' GTACTGCCAT TCGTAACTGT GTACTCAAGT AATGCCTGTC CATCATGTAA ATTGCATGGC CCTGATTGGA TATGCCAAAG GCTTTTGCAA GTCCCCATAG GTACTGGACA GTAGTACGTT GTCCTGAGGC GCGCTATAGG GTCGAAACTG 3 Enter answer. D8 You are creating PCR primers for the DNA sequence pictured. If the given sequence is the plus...
Prepare a fragment of DNA to be cloned by PCR by preparing the oligonucleotides received through...
Prepare a fragment of DNA to be cloned by PCR by preparing the
oligonucleotides received through dilutions. The DNA to be used as
a template is in a 50 ng/ul solution. The protocol is as
follows:
VOLUME TO ADD FINAL CONCENTRATION 0.5 UM 0.5 M REAGENT 10 PM Forward Primer 10 PM Reverse Primer Thermoestable pol Master mix 2x Template DNA water 1x 100 ng.. Total volume 25 ul Information about the primers: Forward primer: 29.3 nmoles - 220 ug...
Forward primer 5'-ACCACCCAGCCCTTAGTGACCAGCTA-3' Reverse primer 5'-AAAGTTTTATTCATTGCAGAGGGGT-3' GAATTCTGTAATAACTATATAGAACTCTTCTTATATATGCTCAAATTTTA CATGCTAGCCTTCAGGTACATATCTTGGGTTGTTGGGTATTGTAGAAGAA TGTACTACAGGGCTTCAGCCCAGTTGACCAATGAGTGGGCTACGGGGTTT GTGAAAGGAGAGATGGAGAAGGAGGGACCATTAAGTACCTTGCTGCCTGA GTTCTGCTTTCCTCCTCCCTCTGAGGGTGAGCTGGGATCTCATCTGAGTT AAGGGCCCAGCTATCAATGGGAACTGTGAAACAGTCCAAGGGACATCAAT ATTAGGTCCCTAACAACTOCAGTTTCCTGGGGAATGATGTGGAAAATGCT CAGCCAAAGATGAAGAAGGTCTCACCTTCTGGGACAATGTCCCCTGCTOG GAACTGGTTCATCAGGCCATCTGGTCCCTTATTAAGACTATAATAACCCT AAGACTAAGTAGATGTGTTGATGTCCAATGAGTGCTTTCTGCAGACCTAG CACCAGGCAAGTGTTTGGAAACTGCAGCTTCAGCCCCTCTGGCCATCTGC CTACCCACCCCACCTGGAGACCTTAATGGGCCAAACAGCAAAGTCCAGGG GGCAGAGAGGAGGTACTTTOGACTATAAAGCTGGTGGGCATCCAGTAACC CCCAGCCCTTAGTGACCAGCTATAATCAGAGACCATCAGCAAGCAGGTAT GTACTCTCCTCTTTGGGCCTGGCTCCCCAGCCAAGACTCCAGOGACTTTA GGGAGAATGTGGGCTCCTCTCTTACATGGATCTTTTGCTAGCCTCAACCC TGCCTATCTTTCAGGTCATTGTTTCAACATGGCCCTGTTGGTGCACTTOC TACCCCTGCTGGCCCTGCTTGCCCTCTGGGAGCCCAAACCCACCCAGGCT TTTGTCAAACAGCATCTTTGTGGTCCCCACCTGGTAGAGGCTCTCTACCT GGTGTGTGGGGAGOGTGGCTTCTTCTACACACCCAAGTOCCGCOGTGAAG TGGAGGACCCACAAGTGGAACAACTGGAGCTGGGAGGAAGCCCOGGGGAC CTTCAGACCTTGGCGTTGGAGGTGGCCOGGCAGAAGCGTGGCATTGTGGA TCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCA ACTAAGGCCCACCTCGACCOGCCCCACCCCTCTGCAATGAATAAAACTIT TGAATAAGCACCAAAAAAAAGAGTTCTATAATGAATGAAAAAGGATIGTG TATATAGACATCTTTTTCTCTGGCATTTATTGTCATGTTAGCATACTATT AAACCATTGTTAGGTTGGATGATTATATAATCATGTATGAAGCTTGTGAT AAAACACCAGGAATAATTCAAGTATCTGGAATTC 3 Question: 1) Predict the sizes of the PCR products. 2) Draw the DNA primers base pairing at genomic sequence.
1. What are the degenerate primers in the PCR amplification protocol? What do they do? 2. Suppose you begin a PCR reaction with 1 piece of double stranded DNA. After 30 cycles of replication, how many pieces of double stranded DNA do you now have? 3. What would be the consequence of having too much DNA in the sample? Would it interfere with the PCR reaction? Why? 4. What happens during the annealing step of the PCR reaction? During the...
please help me!!!!!!!!!
do both i need help
Dz. You are creating PCR primers for the DNA sequence pictured. If the given sequence is the plus strand, what would be the reverse primer? (Type the primer in 5' to 3' orientation without spaces) 5' GTACTGCCAT TCGTAACTGT GTACTCAAGT AATGCCTGTC CATCATGTAA ATTGCATGGC CCTGATTGGA TATGCCAAAG GCTTTTGCAA GTCCCCATAG GTACTGGACA GTAGTACGTT GTCCTGAGGC GCGCTATAGG GTCGAAACTG 3 Enter answer. D8 You are creating PCR primers for the DNA sequence pictured. If the given sequence is the plus...
Prepare a fragment of DNA to be cloned by PCR by preparing the
oligonucleotides received through dilutions. The DNA to be used as
a template is in a 50 ng/ul solution. The protocol is as
follows:
VOLUME TO ADD FINAL CONCENTRATION 0.5 UM 0.5 M REAGENT 10 PM Forward Primer 10 PM Reverse Primer Thermoestable pol Master mix 2x Template DNA water 1x 100 ng.. Total volume 25 ul Information about the primers: Forward primer: 29.3 nmoles - 220 ug...
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