What enzymes are often used to map regions of open chromatin?
What enzymes are often used to map regions of open chromatin?
Homework Answers
Answer: The enzymes which is generally used to map the region of open chromatin is called as Nuclease specially DNase1 and MNase (micrococcal nuclease) .
Explanation: Firstly i like to clear what is chromatin so---
Chromatin = DNA+ Protein means chromatin is the structure where DNA is wrapped around a protein. The protein present here is called as histone protein.
DNase enzyme can act on the DNA strand vigrously irrespective to sequence specificity and can cleave off the DNA strand. When the DNA sequence is bound with protein then in those case there enzymes can not attack the DNA so nuclase DNase 1 is used to cleave the phosphodiester bond between hydroxyl and phosphate group. DNase generally work on SsDNA, DsDNA, as well as in chromatin.
The other type of nuclease is called as micrococcal nuclease(MNase) which is basically acting on single stranded nucleic acid.
Briefly describe three differences between transcriptionally active chromatin regions when compared with other regions of chromatin...
Briefly describe three differences between transcriptionally active chromatin regions when compared with other regions of chromatin that are transcriptionally silent.
What enzymes modify chromatin? How might a transcriptional activator recruit factors that modify chromatin? transcriptional repressor?
What enzymes modify chromatin? How might a transcriptional activator recruit factors that modify chromatin? transcriptional repressor?
In your own words, how do chromatin modifications regulate transcription? What modifications are observed in regions...
In your own words, how do chromatin modifications regulate transcription? What modifications are observed in regions of the genome that are being actively transcribed? In regions that are not actively transcribed?
In eukaryotic transcription what is the function of a chromatin remodeling complex? Chromatin remodeling complexes form...
In eukaryotic transcription what is the function of a chromatin remodeling complex? Chromatin remodeling complexes form nucleosome structures at protein-coding regions to lock genes from transcription when they are inactive. Chromatin remodeling complexes clear nucleosomes away from enhancer regions, so that transcription goes at full rate. Chromatin remodeling complexes reform DNA structure after transcription is complete to keep it stable . Chromatin remodeling complexes clear nucleosomes away from promoter regions, so that transcription initiation complexes can form and function.
Which technique would you use if you wanted to < Identify all the regions of open...
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
1.What is an enzyme? What type of macromolecule are enzymes most often composed of? Using a...
1.What is an enzyme? What type of macromolecule are enzymes most often composed of? Using a chemical equation explain enzyme reactions? Using a diagram explain how enzymes work by lowering activation energy. 2.Why do we attempt prevent the growth of some or all microbes-list several reasons why this might be valuable or necessary? Discuss three methods of physical control of microbes. List 5 different categories of chemical agents used in microbial control and discuss each. What are antibiotics? How have...
What approaches can be used to generate chromatin in vitro? What approach is usually taken to...
What approaches can be used to generate chromatin in vitro? What approach is usually taken to study single nucleosomes on a short DNA fragment, and which is best for assembly arrays of physiologically spaced nucleosomes?
QUESTION 18 Histone modifying enzymes (HATS, HDACs, etc) and chromatin remodeling complexes (SWI/SNF, etc) actively control...
QUESTION 18 Histone modifying enzymes (HATS, HDACs, etc) and chromatin remodeling complexes (SWI/SNF, etc) actively control gene expression. Their actions, in turn, are controlled by o Transcription Factors O DNA Polymerase o Histone H1 Mitochondria
Which molecule is responsible for creating an open chromatin conformation? A. activator B.DNA translocase C. insulator...
Which molecule is responsible for creating an open chromatin conformation? A. activator B.DNA translocase C. insulator D. mediator
What element of replication is used in both prokaryotes and eukaryotes? topoisomerase chromatin assembly factors replication...
What element of replication is used in both prokaryotes and eukaryotes? topoisomerase chromatin assembly factors replication licensing factors shelterin telomerase
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
QUESTION 18 Histone modifying enzymes (HATS, HDACs, etc) and chromatin remodeling complexes (SWI/SNF, etc) actively control gene expression. Their actions, in turn, are controlled by o Transcription Factors O DNA Polymerase o Histone H1 Mitochondria
Most questions answered within 3 hours.
-
7. The term Six Sigma refers to:
Select one:
a. The control limits established in a...
asked 3 minutes ago -
Wildhorse Company follows the practice of pricing its inventory
at the lower-of-cost-or-market, on an individual-item
basis....
asked 5 minutes ago -
For the following reaction: A(g) + 2 B(g) ⇌ 2 C(g) Calculate the
equilibrium constant K...
asked 5 minutes ago -
Which of the following best describes tidal volume? options:
1.The amount of oxygen in the air...
asked 22 minutes ago -
1. Smaller cost distortions occur when the traditional systems'
single indirect-cost rate and the activity-cost-driver rates:...
asked 30 minutes ago -
Using either a FMECA approach or some other appropriate RCA
tool, identify five risk treatment actions...
asked 30 minutes ago -
If for a test the P-value is between 0.0025 and 0.005, what
conclusion can you draw?...
asked 42 minutes ago -
Using Python. Write a function clean2(aList) that takes a list
of integers aList as argument, and...
asked 48 minutes ago -
Intrapreneurship is defined as the
development of an enterprise culture with an existing (Nandan,
2009). An...
asked 1 hour ago -
QUESTION 1 Tamiflu is a drug used to treat influenza. A study
found that Tamiflu reduced...
asked 1 hour ago -
Which of the following would be less likely to be used to
allocate factory overhead when...
asked 1 hour ago -
Transverse pulses travel with a speed of 195 m/s along a taut
copper wire whose diameter...
asked 1 hour ago