What enzymes modify chromatin? How might a transcriptional activator recruit factors that modify chromatin? transcriptional repressor?
Chromatin remodeling is a dynamic modification of chromatin to allow access of condense to genomic DNA to regulatory transcription machinery protein & their by control gene expressions. These remodeling carried out by
*covalent - histone modify complex
* ATP- dependent chromatin
The covalent histone modifications by specific enzymes = histone acrtyltransferases, metyltransferases, kinase.
TRANSCRIPTIONAL ACTIVATOR = Activator is protein that increase gene or set of gene.
TRANSCRIPTIONAL RRPRESSOR= Repressor is a protein that bind to specific DNA sequence & control the expression of gene or operon. Repressor is negativity influenced the ability of RNA POLYMERASE to transcribe the gene or operon.
Repressor bind to small molecule called effector.
Effector are two types= inducer & co-repressor.
Inducer bind to its repressor & co-repressor bind to its repressor. If Co repressor is absent, it does not bind operon & operon can be transcribed by RNA POLYMERASE.
What enzymes modify chromatin? How might a transcriptional activator recruit factors that modify chromatin? transcriptional repressor?
In its active form it turns of gene expression. a.) Transcriptional repressor b.) Transcriptional activator c.) miRNA d.) tRNA e.) terminator
Which is the factor(s) involved in the regulation of gene activity? Select one: a. Transcriptional repressor/activator proteins, which bind to the operator/enhancer sequence regions. b. SAM binding to a riboswitch mRNA. c. Alterations in mRNA conformations by SAM during the transcription. d. All of these. e. None of these.
Mention the components of bacterial transcriptional switches. The Lac operon is regulated by both activator CAP and Lac repressor. A scientist introduces a mutation in the gene encoding the activator CAP, making this allosteric protein insensitive to glucose levels. What outcomes do you expect if you culture the mutated bacteria in a culture medium with glucose and lactose. Give your explanation based on the mechanism of the Lac operon.
Which of the following statements about eukaryotic activator proteins is false? Choose one: A. They stimulate transcriptional initiation by opening up the double helix. * B. They stimulate transcription initiation by recruiting proteins that modify chromatin structure. C. They stimulate transcription initiation by aiding in the assembly of general transcription factors and RNA polymerase at the promoter. D. They stimulate transcription initiation by promoting the assembly of a transcription initiation complex at the promoter.
Consider what you know about the lac operon, and predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels of transcription. and the activator is b. When lactose and glucose are...
discuss the composition and structure of enzymes and discuss the factors that modify enzyme structure and function
Can you please help me fill in the blanks and explain what is happening here? In eukaryotes the enhancer can be 5’ or 3’ of the promoter, and even far away because it can ____________ to the promoter. When it does, the _________________ bound at the enhancer can interact with the RNA polymerase. Transcriptional repressors can compete with transcriptional activators for ________ motifs in the enhancer and thus block binding of the transcriptional activator. It can also mask the ______________between...
What enzymes are often used to map regions of open chromatin?
PLEASE help answer all of question 18 a-e 18. What would the lac operon look like in a eukaryote? List two possible changes in chromatin. Explain them to each other. a. b. Transcriptional control. Draw how a single gene (Lacz) would be transcribed and regulated c. If they are each in different chromosomes, draw how their expression would be coordinated Processing control. Pick one approach and explain to your partners d. Translational regulation. Pick one approach and explain to your...
Where do enzymes come from? What is the difference between a competitive and an allosteric or non competitive inhibitor? What do they inhibit? Would it be effective to create a pharmaceutical product that served as an allosteric or competitive inhibitor? Why? What is an "operon"? Be able to draw and label one. Know where a repressor might attach. Name four factors or processes that contribute to the emergence of antibiotic resistant strains of organisms. (HINT: mutation is not one.) What...