What is the formula for determining the number of CFUS per ml of sample when using...
What is the formula for determining the number of CFUS per ml of sample when using the plate count technique?
Why would you not want to use a plate that has 500 colonies on it?
Why would you not want to centrifuge your sample to mix it?
Homework Answers
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What is the formula for determining the number of CFUS per ml of
sample when using...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony Forming unit) CFU/ml= (Number of Colonies|Dilution factor) (Volume of culture plate) Conclusion: In a narrative form Using the 51 (Chapter 3, Cowan lecture book) Inoculation agar, Incubation, Isolation, Inspection, Identification Use the 5 l-s to explain how it was decided that the infectious southe was or was not the cause The number of colonies in first plate is 79. The number of colonies in...
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume...
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume of culture plate In plate 10-4 there were 150 colonies counted. Experimental process: Pipet 0.25 mL of the S. cerevisiae solution into the test tube labeled 10-1. Pipet the solution up and down several times to ensure all organisms are rinsed from the pipet into the test tube. Pipet 0.25 mL of the 10-1 S. cerevisiae solution into the test tube labeled 10-2. Pipet...
You are trying to determine the approximate bacterial count in a sample of soil. You weigh...
You are trying to determine the approximate bacterial count in a sample of soil. You weigh out 1 gram of soil and add it to 9ml water (tube 1). You then take 1 ml out of tube 1 and place it in 9ml of water in tube 2. Finally, you take 1 ml out of tube 2 and place it into 9 more ml of water in tube 3. You plate out 1.0 ml of the contents of tubes 2...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to...
A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to a bottle containing 99ml of sterile water. You then take 0.1 ml from this dilution bottle and transfer to a second bottle with 0.9 ml of sterile water. Finally, you transfer 1 ml to a Petri dish with nutrient agar. How many colonies would you expect to find on this plate? Would it be countable?
What is the concentration of cells in the starting sample if 1 ml was plated per...
What is the concentration of cells in the starting sample if 1
ml was plated per dilution and the starting sample was river
water?
Report your answer below as a decimal with one significant digit
after the decimal and report the exponent the separate box. See
this example, if your answer is 5.2 x 108 you would
report it as "5.2"
You have the following results from a serial dilution experiment: Sample Dilution Plate Count 1:101 Too many to count...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
Count the number of CFU from each of the plates (record TNTC if there are too...
Count the number of CFU from each of the plates (record TNTC if there are too many to count). Record your counts below and calculate the total number of bacteria per mL and the number of ampicillin-resistant bacteria per mL in the chicken fluid. Show all your calculations in a neat logical manner in the workspace on page 4. Plate Count Plates Without Ampicillin Dilution CFU/Plate Plate # 1 Plate # 2 Average/Plate CFU/mL of Fluid 10-4 52 80 66...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony Forming unit) CFU/ml= (Number of Colonies|Dilution factor) (Volume of culture plate) Conclusion: In a narrative form Using the 51 (Chapter 3, Cowan lecture book) Inoculation agar, Incubation, Isolation, Inspection, Identification Use the 5 l-s to explain how it was decided that the infectious southe was or was not the cause The number of colonies in first plate is 79. The number of colonies in...
What is the concentration of cells in the starting sample if 1
ml was plated per dilution and the starting sample was river
water?
Report your answer below as a decimal with one significant digit
after the decimal and report the exponent the separate box. See
this example, if your answer is 5.2 x 108 you would
report it as "5.2"
You have the following results from a serial dilution experiment: Sample Dilution Plate Count 1:101 Too many to count...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
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