11. Explain how the MIC (minimal inhibitory concentration) can be obtained using the tube dilution test
Homework Answers
Answer: The tube dilution test is the standard method for determining levels of resistance to an antibiotic.
The minimal bactericidal concentration (MBC or MIC) can be determined by subculturing the contents of the tubes onto antibiotic-free solid medium and examining for bacterial growth.
Add Answer to:
11.
Explain how the MIC (minimal inhibitory concentration) can be
obtained using the tube dilution test
How do you perform a minimal inhibitory concentration(MIC) test and a minimal bactericidal concentration (MBC)?
How do you perform a minimal inhibitory concentration(MIC) test and a minimal bactericidal concentration (MBC)?
Does every antibiotic have Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC)?
Does every antibiotic have Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC)?
Select the methods of antimicrobial susceptibility testing that can be used to determine minimal inhibitory concentrations...
Select the methods of antimicrobial susceptibility testing that can be used to determine minimal inhibitory concentrations (MIC). Kirby-Bauer test E-test Tube-dilution test Beta-lactamase testing
The minimum inhibitory concentration, or MIC, refers to the minimum concentration of a chemical that is...
The minimum inhibitory concentration, or MIC, refers to the minimum concentration of a chemical that is required to inhibit the growth of an organism. In the Kirby–Bauer test, which region of the zone of inhibition would represent MIC?
Match the following terminologies related to antimicrobial drugs with their appropriate descriptions. minimal inhibitory concentration (MIC)...
Match the following terminologies related to antimicrobial drugs with their appropriate descriptions. minimal inhibitory concentration (MIC) This term is used to describe compounds that cause greater harm to microbes than to the human host. narrow-spectrum drug This term is used to describe the lowest concentration of a drug effective at preventing growth of particular microbes. selective toxicity This term is used to describe drugs that are effective against only a limited variety of microbes.
Please explain why for 3&4 our diferent-richuring antibic Data Analysis: Tube (Broth) Dilution Test The following...
Please explain why for 3&4
our diferent-richuring antibic Data Analysis: Tube (Broth) Dilution Test The following data were obtained for four different antibiotics (Doom, K.O., Mortum and Steril) by incubating Pseudomonas aeruginosa in a nutrient-rich medium with various concentrations of each antibiotic for 24 hours. This was followed by a subculturing step where each initial culture was used to inoculate a sterile nutrient-rich medium that contained NO antibiotic. Please use the following table to answer questions #1-4 below. (5 points...
QUESTION 2 You performed a Tube-Dilution test to determine MIC and, after incubation, saw no growth...
QUESTION 2 You performed a Tube-Dilution test to determine MIC and, after incubation, saw no growth in all 11 tubes. Which of the following is true? The organismis susceptible to the antibiotic The organism is resistant to the antibiotic The results are inconclusive QUESTION 3 Would the Nitrocefin disk on the left be considered a positive or negative result for Beta-lactamase? RD negative positive
The concentration of a solution after dilution can be found by: where M1 is the concentrated...
The concentration of a solution after dilution can be found
by:
where M1 is the concentrated solution (in
this lab, 0.400 M), and V1 is the volume of the
concentrated solution placed in the test tube. The
V2 is the total volume of
concentrated solution plus water added, and M2
is the concentration of the solution after diluting.
Tube 1 has 2.00 mL of the 0.400 M Ni2+ solution and
8.00 mL of water added. What is the concentration of...
Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the di...
Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the dilute solution. DILUTION OF A STOCK SOLUTION PART C: Measurements and Observations Procedure Check out a 100-mL volumetric flask from your instructor. Fill test tube about 2/3 concentration of the active ingredient from its label including units. Record 1. orlt O " concentration of active ingredient. full of "Magic 2....
how do you calculate the original concentration of a solution using the dilution factor? DF: 6.25...
how do you calculate the original concentration of a solution using the dilution factor? DF: 6.25 Initial ml: 4 Final ml: 25 concentration in diluted sample 11.54 mg/L 46.15 26.15 6.15 76.92
Match the following terminologies related to antimicrobial drugs with their appropriate descriptions. minimal inhibitory concentration (MIC) This term is used to describe compounds that cause greater harm to microbes than to the human host. narrow-spectrum drug This term is used to describe the lowest concentration of a drug effective at preventing growth of particular microbes. selective toxicity This term is used to describe drugs that are effective against only a limited variety of microbes.
Please explain why for 3&4
our diferent-richuring antibic Data Analysis: Tube (Broth) Dilution Test The following data were obtained for four different antibiotics (Doom, K.O., Mortum and Steril) by incubating Pseudomonas aeruginosa in a nutrient-rich medium with various concentrations of each antibiotic for 24 hours. This was followed by a subculturing step where each initial culture was used to inoculate a sterile nutrient-rich medium that contained NO antibiotic. Please use the following table to answer questions #1-4 below. (5 points...
QUESTION 2 You performed a Tube-Dilution test to determine MIC and, after incubation, saw no growth in all 11 tubes. Which of the following is true? The organismis susceptible to the antibiotic The organism is resistant to the antibiotic The results are inconclusive QUESTION 3 Would the Nitrocefin disk on the left be considered a positive or negative result for Beta-lactamase? RD negative positive
The concentration of a solution after dilution can be found
by:
where M1 is the concentrated solution (in
this lab, 0.400 M), and V1 is the volume of the
concentrated solution placed in the test tube. The
V2 is the total volume of
concentrated solution plus water added, and M2
is the concentration of the solution after diluting.
Tube 1 has 2.00 mL of the 0.400 M Ni2+ solution and
8.00 mL of water added. What is the concentration of...
Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the dilute solution. DILUTION OF A STOCK SOLUTION PART C: Measurements and Observations Procedure Check out a 100-mL volumetric flask from your instructor. Fill test tube about 2/3 concentration of the active ingredient from its label including units. Record 1. orlt O " concentration of active ingredient. full of "Magic 2....
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