Question

How do you perform a minimal inhibitory concentration(MIC) test and a minimal bactericidal concentration (MBC)?

Answer 1

MINIMAL INHIBITORY CONCENTRATION:

MINIMUM INHIBITORY CONCENTRATION TEST Cmie) It is a technique used to determine the lowest concentration of a particular antibiotic needed to kill bacteria. PROCEDURE : serial dilutions of the antibiotic (representing different concentrations of the artibistic) are added to a growth medium in reperate test tubes. These tubes are then insulated with the bacteria The tubes are allowed to incubate overnight Broth tubes that appear surbid are indicative of bacterial growth while tubes that remain clear indicate no growth, Mic of the antibiotic is the lowest concentration that does not show growth.

MINIMAL BACTERICIDAL CONCENTRATION:

MINIMUM BACTERICIDAL CONCENTRATION : (MBC) is the lowest concentration of an antibacterial agent required to kill a particular bacterium. PROCEDURES at can be determined from broth dilution mic texts by subculturing to agar plates that do not contain the test agent. The MBC in is identified by determining the lowest concentration of antibacterial agent that reduces the viability a the initial backrial inoculum by z99.94. subculturing and from a not con