Why would it be of interest to put biotin into a secondary antibody (biotinylate it)? What...
Homework Answers
Biotinylated secondary antibodies are used in immunoassay techniques where signal amplification is highly required. Biotin has a very high affinity to bind to streptavidin. Generally, In an immunoassay experiment, as the biotinylated secondary antibodies binds to the target molecule, streptavidin is put, so as to have a high signal amplification.
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Why
would it be of interest to put biotin into a secondary antibody
(biotinylate it)? What...
Why do we use an epitope tag versus a direct antibody for our protein of interest?...
Why do we use an epitope tag versus a direct antibody for our protein of interest? How you would go about making an epitope tag for your favorite gene of interest?
What would you see if you forgot to add the primary antibody? If you forgot the...
What would you see if you forgot to add the primary antibody? If you forgot the secondary antibody? If the blocking agent didn’t work? If you did not permeabilize your cells correctly?
Biochem: what would happen to blood biotin levels if the deficiency was in the holocarboxylase synthetase?
Biochem: what would happen to blood biotin levels if the deficiency was in the holocarboxylase synthetase?
Give an example of a primary group AND a secondary group and explain why you would...
Give an example of a primary group AND a secondary group and explain why you would consider each either a primary or a secondary group. Be sure that you demonstrate an understanding of the differences between a primary and secondary group in your answer.
How would you put this in a financial calculator to get the interest rate
How would you put this in a financial calculator to get the interest rate?This question illustrates what somewhat unscrupulous lender. You want to borrow $29,000 for one year. The interest rate is 19.5 percent. You lender agree that the interest on the loan will be .195x $29,000 $5,655. So, the lender deducts this interest amount from the loan up front and gives you $23,345. In this case, we say that the discount is known as discount interest. Imagine you are...
What is secondary market? Why is secondary market important? Why is development a good, liquid, deep...
What is secondary market? Why is secondary market important? Why is development a good, liquid, deep secondary market important for the economy and the firm?
5. (4 pts) In an immunocytochemical experiment you use a primary antibody against a presy What is...
5. (4 pts) In an immunocytochemical experiment you use a primary antibody against a presy What is your interpretation of this result and what control experiment could you do to determine if the antibody only labels the target protein and not any other off-target protein(s)? (Hint: this control experiment cannot be done in crayfish but only in animals that can be genetically modified, e.g. mouse, Drosophila) naptic protein and you get labeling in an unexpected location (neuronal nuclei).
5. (4...
a. How does one make a humanized antibody? What are the benefits of humanizing an antibody?...
a. How does one make a humanized antibody? What are the benefits of humanizing an antibody? b. Why might there be side effects when an antibody binds to its epitope?
pls help, ty ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
What are some safeguards that you think could be put into place to reduce fear among...
What are some safeguards that you think could be put into place to reduce fear among individuals and groups who oppose identifiable health data collection? Do the benefits of confidential health data collection outweigh the risks? Why or why not? What, according to you, is the purpose of HITECH? What are the new notification requirements? Do you feel these are sufficient? Why or why not? What possible HIPAA violations could occur with portable PHI? What strategies should be put into...
5. (4 pts) In an immunocytochemical experiment you use a primary antibody against a presy What is your interpretation of this result and what control experiment could you do to determine if the antibody only labels the target protein and not any other off-target protein(s)? (Hint: this control experiment cannot be done in crayfish but only in animals that can be genetically modified, e.g. mouse, Drosophila) naptic protein and you get labeling in an unexpected location (neuronal nuclei).
5. (4...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
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