A congenital defect in the liver enzyme fructose-1,6-bisphosphatase may result in metabolic acidosis. Explain.
mechanism of action of the enzyme fructose 1,6-bisphosphatase? how is the enzyme regulated
what forces stabilize secondary, tertiary, and possible quaternary structure of Fructose 1,6 bisphosphatase? where is the catalytic site and what is the ionization state the side chains at the optimal pH for this enzyme?
Explain in detail how PFK-1, hexokinase, glucokinase, pyruvate kinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase-1, pyruvate carboxylase & PEPCK are regulated & compare their regulation in liver vs. skeletal muscle & brain when blood glucose levels are high (i.e. a fed state) & low (i.e. a fasting or starvation state).
BIOCHEM Compare and contrast how fructose‐1,6‐bisphosphatase and phosphofructokinase are regulated in response to energy charge. What specific allosteric effectors are involved? What is meant by reciprocal regulation. Pyruvate kinase is also regulated allosterically by fructose‐1,6‐bisphosphate. Explain the rationale for this regulation. Do these compounds stimulate or inhibit pyruvate kinase?
match the following 1. Fructose 1,6-bisphosphate is converted to Fructose 6- phosphate through this enzyme _____ 2. Synthesis of glucose from noncarbohydrate precursors _____ 3. Stage in which two molecules of ATP are consumed _____ 4. This is formed from pyruvate in yeast and several other microorganisms through fermentation _____ 5. The isomerization of glucose 6-phosphate to fructose 6-phosphate is a coversion of an aldose into a …._____ 6. Pyruvate is converted by pyruvate carboxylase into this intermediate _____ 7....
What metabolic defect results from impairment of the enzyme homogentisate oxidase in the tyrosine catabolic pathway? Please explain the defect and common symptoms.
Concentrations of some glycolysis intermediates in the red blood cell (mM): Glucose Glucose-6-P Fructose-6-P Fructose-1,6-BP 1,3-bisphosphoglycerate 0.001 3-phosphoglycerate 5.0 0.083 0.014 0.031 2- phosphoglycerate Phosphoenolpyruvate Pyruvate ATP ADP Pi 0.030 0.023 0.051 1.85 0.14 1.0 0.12 3. Isotopic labeling (incorporating a rare isotope, eg. the radioactive 1C, into a molecule at a defined position) is an important technique for studying metabolism as it enables us to follow individual atoms through a metabolic pathway 2 a. If 4CO2 were bubbled through...
please explain answer Regulation of Lactate dehydrogenase (same enzyme described above). Lactose dehydrogenase (LDH) is a tetramer that can be regulated in several different ways. The crystal structure of the four subunits of lactate dehydrogenase is shown below. In the heart, lactose has a higher affinity for pyruvate than in the skeletal muscle. Analysis of LDH has identified that it is a substrate of FGFR tyrosine kinase, which results in an increase in the production of lactate. Some types of...