You have a tube with 3 ml of lymphocytes that are at a concentration of 7 x 106 cells/ml. You also have a supply of cell culture media that can be used to dilute cells. You are asked to make up an 8 ml sample with a lymphocyte concentration of 3 x 105 cells/ml. How will you achieve this? Show your calculations and then write your answer.
Ans: Use a dilution equation to solve this type of problems
Dilution equation is C1V1 = C2V2
Here,
C1 = 7 x 106 cells/ml (7000000 cells/ml)
V1 = 3 ml
C2 = 3 x 105 cells/ml (300000 cells/ml)
V2 = first we need to find out
to calculate V2
we use V2 = C1V1 / C2
V2 = (7000000 X 3) / 300000
V2 = 70 ml
When you add 67 ml of cell culture media to 3 ml of 7 x 106 cells/ml
You can get 70 ml of 3 x 105 cells/ml
From the above 70ml solution you can collect 8 ml of solution which contains 3 x 105 lymphocytes in each 1ml of cell culture media
If you want to reduce use of cell culture media, Then you can calculate it by following way
In this case,
C1 = 7 x 106 cells/ml (7000000 cells/ml)
V1 = we nned to find this to reduce usage of culture media
C2 = 3 x 105 cells/ml (300000 cells/ml)
V2 = 8 ml
Hence, V1 = C2V2 / C1
V1 = (300000 X 8) / 7000000
V1 = 0.3428 ml
If you make up 0.3428 ml of lymphocytes at a concentration of 7 x 106 cells/ml to 8ml of volume you can get concentration of 3 x 105 cells/ml
You have a tube with 3 ml of lymphocytes that are at a concentration of 7...
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