You have a stock solution of A. pasteurianus at a concentration of 5 x 108 cfu/ml. If you take 10µl of this stock solution and place it in 990µl of sterile broth culture media, what is the concentration of cells in the broth culture?
You have a stock solution of A. pasteurianus at a concentration of 5 x 108 cfu/ml....
you are given three stock cultures- E coli at a concentration of 1.5 x 10^3 cfu/ml, Bacillus at a concentration of 4.2 x10^3 cfu/ml, and staphylococcus at a concentratino of 2.4 x10^4 cful/ml. you ahve been asked to make a 30 ml of a single mixed culture with a final concentration of 500 cfu/ml of E coli, 150 cfu/ml of bacillus, and 80 cfu/ml of Staph. How would you make this mixture?
If you have a stock culture of bacteria at a concentration of 5*10 cells/ml, how much stock cuiture and how much media would you add to obtain a final concentration of 2.5*10 cells/ml in a final volume of 20mL? (1 point)
c)You have 0.6 mL an undiluted culture at a density of 3.7 x 107 CFU/mL. You add 5.4 mL of sterile diluent. What is the dilution and final cell density? e) Plating 2.0 mL of a sample diluted by a factor or 10-2 procedures 56 colonies. What was the original cell density in the sample? f)Plating 0.2 mL of a sample diluted by a factor or 10-4 procedures 87 colonies. What was the original cell density in the sample?
You have a tube with 3 ml of lymphocytes that are at a concentration of 7 x 106 cells/ml. You also have a supply of cell culture media that can be used to dilute cells. You are asked to make up an 8 ml sample with a lymphocyte concentration of 3 x 105 cells/ml. How will you achieve this? Show your calculations and then write your answer.
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
You calculate based on A600 that a suspension contains 2.04 x 108 CFU ml-1 How many CFU are in the suspension? 204 x 101 o 2.04 x 102 2.04 x 103 2.04 x 104 2.04 x 105 2.04 x 106