If you have a stock culture of bacteria at a concentration of 5*10 cells/ml, how much...

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Answer 1

Answer #1

According to the formula:

M1V1 = M2V2,

we have:

Stock solution concentration: 5*10⁸ cells/ml

Required solution concentration: 2.5*10⁶ cells/ml

Required volume: 20 ml

Thus, the required volume of stock can be calculated as:

V1 = M2V2/M1

V1 = 2.5*10⁶ * 20 / 5*10⁸

V1 = 50 * 10⁶ / 5 * 10⁸

V1 = 0.1 ml

Thus, 100ul of stock must be diluted to 20 ml in order to obtain a 5*10⁶ cells/ml.

Answer 2

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